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Data from Central South University Provide New Insights into Cytometry (In vivo and in vitro evaluation of the cytotoxic effects of Photosan-loaded...

August 19, 2014



Data from Central South University Provide New Insights into Cytometry (In vivo and in vitro evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer)

By a News Reporter-Staff News Editor at Cancer Weekly -- Current study results on Cytometry have been published. According to news reporting from Changsha, People's Republic of China, by NewsRx journalists, research stated, "This study aimed to compare the inhibitory effects of photosensitizers loaded in hollow silica nanoparticles and conventional photosensitizers on HepG2 human hepatoma cell proliferation and determine the underlying mechanisms. Photosensitizers (conventional Photosan-II or nanoscale Photosan-II) were administered to in vitro cultured HepG2 hepatoma cells and treated by photodynamic therapy (PDT) with various levels of light exposure."

The news correspondents obtained a quote from the research from Central South University, "To assess photosensitizers' effects, cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, apoptotic and necrotic cells were measured by flow cytometry and the expression of caspase-3 and caspase-9 evaluated by western blot. Finally, the in vivo effects of nanoscale and conventional photosensitizers on liver cancer were assessed in nude mice. Nanoscale Photosan-II significantly inhibited hepatoma cell viability in a concentration-dependent manner and this effect was more pronounced with high laser doses. Moreover, nanoscale photosensitizers performed better than the conventional ones under the same experimental conditions (p < 0.05). Flow cytometry data demonstrated that laser-induced cell death was markedly increased after treatment with nanoscale Photosan-II in comparison with free Photosan-II (p < 0.05). Activated caspase-3 and caspase-9 levels were significantly higher in cells treated with Photosan-II loaded in silica nanoparticles than free Photosan-II (p < 0.05). Accordingly, treatment with nanoscale photosensitizers resulted in improved outcomes (tumor volume) in a mouse model of liver cancer, in comparison with conventional photosensitizers."

According to the news reporters, the research concluded: "Hollow silica nanoparticles containing photosensitizer more efficiently inhibited hepatoma cells than photosensitizer alone, through induction of apoptosis, both in vivo and in vitro."

For more information on this research see: In vivo and in vitro evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer. Nanoscale Research Letters, 2014;9():1-8. Nanoscale Research Letters can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Nanoscale Research Letters - www.springerlink.com/content/1931-7573/)

Our news journalists report that additional information may be obtained by contacting Z.T. Liu, Central South University, State Key Lab Powder Met, Changsha 410083, Hunan, People's Republic of China. Additional authors for this research include L. Xiong, Z.P. Liu, X.Y. Miao, L.W. Lin and Y. Wen (see also Cytometry).

Keywords for this news article include: Asia, Cancer, Changsha, Oncology, Cytometry, Nanoscale, Nanoparticle, Nanotechnology, Emerging Technologies, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Cancer Weekly


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