Study Findings from University of Missouri Broaden Understanding of Green Fluorescent Protein (Decorin-PEI nanoconstruct attenuates equine corneal fibroblast differentiation)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Proteins. According to news reporting originating from Columbia, Missouri, by NewsRx correspondents, research stated, "To explore (i) the potential of polyethylenimine (PEI) nanoparticles as a vector for delivering genes into equine corneal fibroblasts (ECFs) using green fluorescent protein (GFP) marker gene, (ii) whether PEI nanoparticle-mediated decorin (DCN) gene therapy could be used to inhibit fibrosis in the equine cornea using an in vitro model. Procedure Polyethylenimine-DNA nanoparticles were prepared at nitrogen-to-phosphate (N-P) ratio of 15 by mixing 22kDa linear PEI and a plasmid encoding either GFP or DCN."
Our news editors obtained a quote from the research from the University of Missouri, "ECFs were generated from donor corneas as previously described. Initially, GFP was introduced into ECFs using PEI nanoparticles to confirm gene delivery, then DCN was introduced to evaluate for antifibrotic effects. GFP gene delivery was confirmed with real-time qPCR and ELISA. Changes in fibrosis after DCN therapy were quantified by measuring -smooth muscle actin (SMA) mRNA and protein levels with qPCR, immunostaining, and immunoblotting. Cytotoxicity was determined by evaluating cell morphology, cellular viability, and TUNEL assay. Polyethylenimine-green fluorescent protein-treated cultures showed 2.2x104 GFP plasmid copies/g of cellular DNA and 2.1pg of GFP/100L of lysate. PEI-DCN delivery significantly attenuated TGF-induced transdifferentiation of fibroblasts to myofibroblasts (2-fold decrease of SMA mRNA; P=0.05) and significant inhibition of SMA (49 +/- 14.2%; P
According to the news editors, the research concluded: "PEI-mediated DCN gene delivery is effective at inhibiting TGF-mediated fibrosis in this model."
For more information on this research see: Decorin-PEI nanoconstruct attenuates equine corneal fibroblast differentiation. Veterinary Ophthalmology, 2014;17(3):162-169. Veterinary Ophthalmology can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Veterinary Ophthalmology - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1463-5224)
The news editors report that additional information may be obtained by contacting K.S. Donnelly, University of Missouri, Mason Eye Inst, Columbia, MO 65212, United States. Additional authors for this research include E.A. Giuliano, A. Sharma, A. Tandon, J.T. Rodier and R.R. Mohan (see also Proteins).
Keywords for this news article include: Therapy, Columbia, Missouri, Fibroblasts, Nanoparticle, United States, Nanotechnology, Emerging Technologies, Connective Tissue Cells, North and Central America, Green Fluorescent Protein
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC