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Study Data from Northwestern University Update Knowledge of Tissue Engineering (A PLG/HAp composite scaffold for lentivirus delivery)

July 17, 2014

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Data detailed on Biomedicine and Biomedical Engineering have been presented. According to news reporting from Evanston, Illinois, by NewsRx journalists, research stated, "Gene delivery from tissue engineering scaffolds provides the opportunity to control the microenvironment by inducing expression of regenerative factors. Hydroxyapatite (HAp) nanoparticles can bind lentivirus, and we investigated the incorporation of HAp into poly(lactide-co-glycolide) (PLG) scaffolds in order to retain lentivirus added to the scaffold."

The news correspondents obtained a quote from the research from Northwestern University, "PLG/HAp scaffolds loaded with lentivirus enhanced transgene expression over 10-fold in vitro relative to scaffolds without HAp. Following in vivo implantation, PLG/HAp scaffolds promoted transgene expression for more than 100 days, with the level and duration enhanced relative to control scaffolds with lentivirus/HAp complexes added to PLG scaffolds. The extent of HAp incorporated into the scaffold influenced transgene expression, in part through its impact on porous architecture. Expression in vivo was localized to PLG/HAp scaffolds, with macrophages the primary cell type transduced at day 3, yet transduction of neutrophils and dendritic cells was also observed. At day 21 in PLG/HAp scaffolds, non-immune cells were transduced to a greater extent than immune cells, a trend that was opposite results from PLG scaffolds."

According to the news reporters, the research concluded: "Thus, in addition to retaining the virus, PLG/HAp influenced cell infiltration and preferentially transduced non-immune cells."

For more information on this research see: A PLG/HAp composite scaffold for lentivirus delivery. Biomaterials, 2013;34(21):5431-8. (Elsevier -; Biomaterials -

Our news journalists report that additional information may be obtained by contacting R.M. Boehler, Dept. of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208-3120, United States. Additional authors for this research include S. Shin, A.G. Fast, R.M. Gower and L.D Shea (see also Biomedicine and Biomedical Engineering).

Keywords for this news article include: Tissue Engineering, Biomedicine and Biomedical Engineering, Evanston, Illinois, United States, Bioengineering, North and Central America.

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Source: Gene Therapy Weekly

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