Studies from Lomonosov Moscow State University Further Understanding of Mosaic Viruses (Complexes assembled from TMV-derived spherical particles and entire virions of heterogeneous nature)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on RNA Viruses. According to news reporting out of Moscow, Russia, by NewsRx editors, research stated, "Previously, we described some structural features of spherical particles (SPs) generated by thermal remodelling of the tobacco mosaic virus. The SPs represent a universal platform that could bind various proteins."
Our news journalists obtained a quote from the research from Lomonosov Moscow State University, "Here, we report that entire isometric virions of heterogeneous nature bind non-specifically to the SPs. Formaldehyde (FA) was used for covalent binding of a virus to the SPs surface for stabilizing the SP-virus complexes. Transmission and high resolution scanning electron microscopy showed that the SPs surface was covered with virus particles. The architecture of SP-virion complexes was examined by immunologic methods. Mean diameters of SPs and SP-human enterovirus C and SP-cauliflower mosaic virus (CaMV) compositions were determined by nanoparticle tracking analysis (NTA) in liquid. Significantly, neither free SPs nor individual virions were detected by NTA in either FA-crosslinked or FA-untreated compositions. Entirely, all virions were bound to the SPs surface and the SP sites within the SP-CaMV complexes were inaccessible for anti-SP antibodies. Likewise, the SPs immunogenicity within the FA-treated SPs-CaMV compositions was negligible. Apparently, the SP antigenic sites were hidden and masked by virions within the compositions. Previously, we reported that the SPs exhibited adjuvant activity when foreign proteins/epitopes were mixed with or crosslinked to SPs. We found that immunogenicity of entire CaMV crosslinked to SP was rather low which could be due to the above-mentioned masking of the SPs booster."
According to the news editors, the research concluded: "Contrastingly, immunogenicity of the FA-untreated compositions increased significantly, presumably, due to partial release of virions and unmasking of some SPs-buster sites after animals immunization."
For more information on this research see: Complexes assembled from TMV-derived spherical particles and entire virions of heterogeneous nature. Journal of Biomolecular Structure & Dynamics, 2014;32(8):1193-201 (see also RNA Viruses).
Our news journalists report that additional information may be obtained by contacting E. Trifonova, a Dept. of Virology , Lomonosov Moscow State University , 1, 12 Leninskie gory, Moscow 119991 , Russia. Additional authors for this research include N. Nikitin, A. Gmyl, E. Lazareva, O. Karpova and J. Atabekov.
Keywords for this news article include: Moscow, Russia, Eurasia, RNA Viruses, Plant Viruses, Mosaic Viruses.
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