Studies from Jawaharlal Nehru University in the Area of Molecular Biotechnology Reported (Transgenic Brassica juncea plants expressing MsrA1, a synthetic cationic antimicrobial peptide, exhibit resistance to fungal phytopathogens)
By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Biotechnology are discussed in a new report. According to news reporting originating from New Delhi, India, by NewsRx correspondents, research stated, "Cationic antimicrobial peptides (CAPs) have shown potential against broad spectrum of phytopathogens. Synthetic versions with desirable properties have been modeled on these natural peptides."
Our news editors obtained a quote from the research from Jawaharlal Nehru University, "MsrA1 is a synthetic chimera of cecropin A and melittin CAPs with antimicrobial properties. We generated transgenic Brassica juncea plants expressing the msrA1 gene aimed at conferring fungal resistance. Five independent transgenic lines were evaluated for resistance to Alternaria brassicae and Sclerotinia sclerotiorum, two of the most devastating pathogens of B. juncea crops. In vitro assays showed inhibition by MsrA1 of Alternaria hyphae growth by 44-62 %. As assessed by the number and size of lesions and time taken for complete leaf necrosis, the Alternaria infection was delayed and restricted in the transgenic plants with the protection varying from 69 to 85 % in different transgenic lines. In case of S. sclerotiorum infection, the lesions were more severe and spread profusely in untransformed control compared with transgenic plants. The sclerotia formed in the stem of untransformed control plants were significantly more in number and larger in size than those present in the transgenic plants where disease protection of 56-71.5 % was obtained."
According to the news editors, the research concluded: "We discuss the potential of engineering broad spectrum biotic stress tolerance by transgenic expression of CAPs in crop plants."
For more information on this research see: Transgenic Brassica juncea plants expressing MsrA1, a synthetic cationic antimicrobial peptide, exhibit resistance to fungal phytopathogens. Molecular Biotechnology, 2014;56(6):535-45. Molecular Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Molecular Biotechnology - www.springerlink.com/content/1073-6085/)
The news editors report that additional information may be obtained by contacting A. Rustagi, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Additional authors for this research include D. Kumar, S. Shekhar, M.A. Yusuf, S. Misra and N.B Sarin (see also Biotechnology).
Publisher contact information for the journal Molecular Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Asia, Biotechnology, India, New Delhi.
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