Studies from Embrapa Have Provided New Information about Applied microbiology and biotechnology (Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database)
The news reporters obtained a quote from the research from Embrapa, "Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning to mass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated from grape must and 67.7 % of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After matching the correct taxon for these strains by using molecular biology approaches, the spectra concerning the missing species were added in a supplementary database."
According to the news reporters, the research concluded: "This new library was able to accurately predict unidentified species at first instance by MALDI-TOF MS, proving it is a powerful tool for the identification of environmental yeasts."
For more information on this research see: Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database. Applied Microbiology and Biotechnology, 2014;98(12):5645-5654. Applied Microbiology and Biotechnology can be contacted at: Springer,
Our news correspondents report that additional information may be obtained by contacting
Keywords for this news article include: Biotechnology,
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