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Studies from Embrapa Have Provided New Information about Applied microbiology and biotechnology (Evaluation of MALDI-TOF mass spectrometry for...

July 16, 2014



Studies from Embrapa Have Provided New Information about Applied microbiology and biotechnology (Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database)

By a News Reporter-Staff News Editor at Biotech Week -- New research on Biotechnology is the subject of a report. According to news reporting originating in Brasilia, Brazil, by NewsRx journalists, research stated, "Yeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand."

The news reporters obtained a quote from the research from Embrapa, "Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning to mass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated from grape must and 67.7 % of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After matching the correct taxon for these strains by using molecular biology approaches, the spectra concerning the missing species were added in a supplementary database."

According to the news reporters, the research concluded: "This new library was able to accurately predict unidentified species at first instance by MALDI-TOF MS, proving it is a powerful tool for the identification of environmental yeasts."

For more information on this research see: Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database. Applied Microbiology and Biotechnology, 2014;98(12):5645-5654. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)

Our news correspondents report that additional information may be obtained by contacting B.C. Agustini, EMBRAPA Recursos Genet & Biotecnol, LEM, Brasilia, DF, Brazil. Additional authors for this research include L.P. Silva, C. Bloch, T.M.B. Bonfim and G.A. da Silva (see also Biotechnology).

Keywords for this news article include: Biotechnology, Brazil, Brasilia, South America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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