Studies from Children's Hospital Provide New Data on Nanoparticles (Site-specific gene delivery to stented arteries using magnetically guided zinc oleate-based nanoparticles loaded with adenoviral vectors)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Nanoparticles are presented in a new report. According to news originating from Philadelphia, Pennsylvania, by NewsRx correspondents, research stated, "Gene therapeutic strategies have shown promise in treating vascular disease. However, their translation into clinical use requires pharmaceutical carriers enabling effective, site-specific delivery as well as providing sustained transgene expression in blood vessels."
Our news journalists obtained a quote from the research from Children's Hospital, "While replication-deficient adenovirus (Ad) offers several important advantages as a vector for vascular gene therapy, its clinical applicability is limited by rapid inactivation, suboptimal transduction efficiency in vascular cells, and serious systemic adverse effects. We hypothesized that novel zinc oleate-based magnetic nanoparticles (MNPs) loaded with Ad would enable effective arterial cell transduction by shifting vector processing to an alternative pathway, protect Ad from inactivation by neutralizing factors, and allow site-specific gene transfer to arteries treated with stent angioplasty using a 2-source magnetic guidance strategy. Ad-loaded MNPs effectively transduced cultured endothelial and smooth muscle cells under magnetic conditions compared to controls and retained capacity for gene transfer after exposure to neutralizing antibodies and lithium iodide, a lytic agent causing disruption of free Ad. Localized arterial gene expression significantly stronger than in control animal groups was demonstrated after magnetically guided MNP delivery in a rat stenting model 2 and 9 d post-treatment, confirming feasibility of using Ad-loaded MNPs to achieve site-specific transduction in stented blood vessels."
According to the news editors, the research concluded: "Ad-loaded MNPs formed by controlled precipitation of zinc oleate represent a novel delivery system, well-suited for efficient, magnetically targeted vascular gene transfer."
For more information on this research see: Site-specific gene delivery to stented arteries using magnetically guided zinc oleate-based nanoparticles loaded with adenoviral vectors. The Faseb Journal, 2013;27(6):2198-206 (see also Nanoparticles).
The news correspondents report that additional information may be obtained from M. Chorny, Division of Cardiology, The Children's Hospital of Philadelphia, Philadelphia, PA 19104-4318, United States. Additional authors for this research include I. Fishbein, J.E. Tengood, R.F. Adamo, I.S. Alferiev and R.J Levy.
Keywords for this news article include: Philadelphia, Pennsylvania, United States, Nanotechnology, Emerging Technologies, North and Central America.
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