Researchers' Work from Jimei University Focuses on Peroxidases (Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Enzymes and Coenzymes have been published. According to news reporting originating from Xiamen, People's Republic of China, by NewsRx correspondents, research stated, "In this paper, Au nanoparticles (AuNPs) have been homogeneously deposited on citrate-functionalized graphene nanosheets (Cit-GNs) by a simple one-pot reducing method. The morphology and composition of the thus-prepared AuNPs/Cit-GNs were characterized by transmission electron microscopy (TEM), high resolution TEM, energy dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy."
Our news editors obtained a quote from the research from Jimei University, "The results showed that the AuNPs with a uniform size are well dispersed on the surface of the Cit-GNs. Significantly, the as-prepared AuNPs/Cit-GNs possess intrinsic peroxidase-like activity, which can catalyze the oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) to develop a blue color in aqueous solution. The catalysis was in accordance with Michaelis-Menten kinetics and the AuNPs/Cit-GNs showed a strong affinity for both H2O2 and TMB. Moreover, by comparing with Cit-AuNPs, AuNPs/GNs and AuNPs/PVP-GNs, the AuNPs/Cit-GNs composite exhibits a higher catalytic ability with a lower Michaelis constant (Km) value, suggesting that the GNs with a large surface area and the citrate ions with more carboxyl groups around the AuNPs can greatly enhance the peroxidase-like activity of AuNPs/Cit-GNs."
According to the news editors, the research concluded: "Taking the advantages of the high catalytic activity, the good stability and the low cost, the novel AuNPs/Cit-GNs represent a promising candidate as an enzyme mimic and may find a wide range of new applications in biochemistry and biotechnology."
For more information on this research see: Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance. Dalton Transactions, 2014;43(20):7449-54. (Royal Society of Chemistry - www.rsc.org/; Dalton Transactions - pubs.rsc.org/en/journals/journalissues/dt)
The news editors report that additional information may be obtained by contacting X. Chen, College of Biological Engineering, Jimei University, Xiamen, 361021, People's Republic of China. Additional authors for this research include X. Tian, B. Su, Z. Huang, X. Chen and M. Oyama (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Xiamen, Nanosheets, Peroxidases, Nanoparticle, Nanotechnology, Oxidoreductases, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China.
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