Research Results from University of California Update Knowledge of Enzymes and Coenzymes (Electronic measurements of single-molecule catalysis by cAMP-dependent protein kinase A)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Enzymes and Coenzymes is now available. According to news reporting originating in Irvine, California, by NewsRx journalists, research stated, "Single-molecule studies of enzymes open a window into their dynamics and kinetics. A single molecule of the catalytic domain of cAMP-dependent protein kinase A (PKA) was attached to a single-walled carbon nanotube device for long-duration monitoring."
The news reporters obtained a quote from the research from the University of California, "The electronic recording clearly resolves substrate binding, ATP binding, and cooperative formation of PKA's catalytically functional, ternary complex. Using recordings of a single PKA molecule extending over 10 min and tens of thousands of binding events, we determine the full transition probability matrix and conversion rates governing formation of the apo, intermediate, and closed enzyme configurations. We also observe kinetic rates varying over 2 orders of magnitude from one second to another. Anti-correlation of the on and off rates for PKA binding to the peptide substrate, but not ATP, demonstrates that regulation of enzyme activity results from altering the stability of the PKA-substrate complex, not its binding to ATP."
According to the news reporters, the research concluded: "The results depict a highly dynamic enzyme offering dramatic possibilities for regulated activity, an attribute useful for an enzyme with crucial roles in cell signaling."
For more information on this research see: Electronic measurements of single-molecule catalysis by cAMP-dependent protein kinase A. Journal of the American Chemical Society, 2013;135(21):7861-8. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)
Our news correspondents report that additional information may be obtained by contacting P.C. Sims, Dept. of Physics and Astronomy, University of California, Irvine, California 92697, United States. Additional authors for this research include I.S. Moody, Y. Choi, C. Dong, M. Iftikhar, B.L. Corso, O.T. Gul, P.G. Collins and G.A Weiss (see also Enzymes and Coenzymes).
Keywords for this news article include: Irvine, California, United States, Protein Kinases, Enzymes and Coenzymes, North and Central America, Phosphotransferases (Alcohol Group Acceptor).
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