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Research Conducted by M.L. Le Brocq and Co-Authors Has Provided New Information about Inflammation Mediators (Chemokines as Novel and Versatile...

July 16, 2014



Research Conducted by M.L. Le Brocq and Co-Authors Has Provided New Information about Inflammation Mediators (Chemokines as Novel and Versatile Reagents for Flow Cytometry and Cell Sorting)

By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biological Factors. According to news reporting from Surrey, United Kingdom, by NewsRx journalists, research stated, "Cell therapy regimens are frequently compromised by low-efficiency cell homing to therapeutic niches. Improvements in this regard would enhance effectiveness of clinically applicable cell therapy."

The news correspondents obtained a quote from the research, "The major regulators of tissue-specific cellular migration are chemokines, and therefore selection of therapeutic cellular populations for appropriate chemokine receptor expression would enhance tissue-homing competence. A number of practical considerations preclude the use of Abs in this context, and alternative approaches are required. In this study, we demonstrate that appropriately labeled chemokines are at least as effective in detecting their cognate receptors as commercially available Abs. We also demonstrate the utility of biotinylated chemokines as cell-sorting reagents. Specifically, we demonstrate, in the context of CCR7 (essential for lymph node homing of leukocytes), the ability of biotinylated CCL19 with magnetic bead sorting to enrich for CCR7-expressing cells. The sorted cells demonstrate improved CCR7 responsiveness and lymph node-homing capability, and the sorting is effective for both T cells and dendritic cells. Importantly, the ability of chemokines to detect CCR7, and sort for CCR7 positivity, crosses species being effective on murine and human cells. This novel approach to cell sorting is therefore inexpensive, versatile, and applicable to numerous cell therapy contexts."

According to the news reporters, the research concluded: "We propose that this represents a significant technological advance with important therapeutic implications."

For more information on this research see: Chemokines as Novel and Versatile Reagents for Flow Cytometry and Cell Sorting. Journal of Immunology, 2014;192(12):6120-6130. Journal of Immunology can be contacted at: Amer Assoc Immunologists, 9650 Rockville Pike, Bethesda, MD 20814, USA. (The American Association of Immunologists - www.aai.org; Journal of Immunology - www.jimmunol.org)

Our news journalists report that additional information may be obtained by contacting M.L. Le Brocq, Miltenyi Biotec, Clin Sci & Cell Anal Grp, Bisley GU24 9DR, Surrey, United Kingdom. Additional authors for this research include A.R. Fraser, G. Cotton, K. Woznica, C.V. McCulloch, K.D. Hewit, C.S. McKimmie, R.J.B. Nibbs, J.D.M. Campbell and G.J. Graham (see also Biological Factors).

Keywords for this news article include: Biotechnology, Surrey, Europe, Cytometry, Immunology, Cell Therapy, Therapeutics, United Kingdom, Membrane Proteins, Biological Factors, Biological Therapy, Cytokine Receptors, Chemokine Receptors, Chemotactic Factors, Inflammation Mediators, G-Protein-Coupled Receptors

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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