Reports Outline Transcription Factors Findings from University of Kansas (Controlling for Gene Expression Changes in Transcription Factor Protein Networks)
By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteins are discussed in a new report. According to news originating from Kansas City, Kansas, by NewsRx correspondents, research stated, "The development of affinity purification technologies combined with mass spectrometric analysis of purified protein mixtures has been used both to identify new protein-protein interactions and to define the subunit composition of protein complexes. Transcription factor protein interactions, however, have not been systematically analyzed using these approaches."
Our news journalists obtained a quote from the research from the University of Kansas, "Here, we investigated whether ectopic expression of an affinity tagged transcription factor as bait in affinity purification mass spectrometry experiments perturbs gene expression in cells, resulting in the false positive identification of bait-associated proteins when typical experimental controls are used. Using quantitative proteomics and RNA sequencing, we determined that the increase in the abundance of a set of proteins caused by overexpression of the transcription factor RelA is not sufficient for these proteins to then co-purify non-specifically and be mis-identified as bait-associated proteins. Therefore, typical controls should be sufficient, and a number of different baits can be compared with a common set of controls. This is of practical interest when identifying bait interactors from a large number of different baits. As expected, we found several known RelA interactors enriched in our RelA purifications (NF kappa B1, NF kappa B2, Rel, RelB, I kappa B alpha, I kappa B beta, and I kappa B epsilon). We also found several proteins not previously described in association with RelA, including the small mitochondrial chaperone Tim13. Using a variety of biochemical approaches, we further investigated the nature of the association between Tim13 and NF kappa B family transcription factors."
According to the news editors, the research concluded: "This work therefore provides a conceptual and experimental framework for analyzing transcription factor protein interactions."
For more information on this research see: Controlling for Gene Expression Changes in Transcription Factor Protein Networks. Molecular & Cellular Proteomics, 2014;13(6):1510-1522. Molecular & Cellular Proteomics can be contacted at: Amer Soc Biochemistry Molecular Biology Inc, 9650 Rockville Pike, Bethesda, MD 20814-3996, USA. (American Society for Biochemistry and Molecular Biology - www.asbmb.org; Molecular & Cellular Proteomics - www.mcponline.org/)
The news correspondents report that additional information may be obtained from C.A.S. Banks, University of Kansas, Medical Center, Dept. of Lab Med, Kansas City, KS 66160, United States. Additional authors for this research include Z.T. Lee, G. Boanca, M. Lakshminarasimhan, B.D. Groppe, Z.H. Wen, G.L. Hattem, C.W. Seidel, L. Florens and M.P. Washburn (see also Proteins).
Keywords for this news article include: Proteins, Kansas City, United States, Transcription Factors, North and Central America
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