Reports from Food and Drug Administration Provide New Insights into Industrial Biotechnology (Evaluation of liquid and solid culture media for the recovery and enrichment of Burkholderia cenocepacia from distilled water)
By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting from Beltsville, Maryland, by NewsRx journalists, research stated, "Burkholderia cepacia complex (BCC) presence has been the cause of recalls of both sterile and non-sterile pharmaceutical products since these opportunistic pathogens have been implicated to cause infections to susceptible individuals. BCC are ubiquitous in nature, but in pharmaceutical settings the most common source is contaminated water systems."
The news correspondents obtained a quote from the research from Food and Drug Administration, "Some strains of BCC, previously described as Pseudomonas cepacia, were not readily detected by standard culture methods. We have explored different strategies to recover and enrich Burkholderia cenocepacia previously cultured in distilled water for 40 days. Enrichment media of varied nutrient concentrations and composition were used, including modified Tryptic Soy Agar or Broth (TSA or TSB), Reasoner's 2nd Agar or Broth (R2A or R2AB), Brain-Heart Infusion Broth (BHIB), Mueller-Hinton Broth (MHB), and Ashdown's (ASH) medium. Of the various broth media tested, cell growth was significantly greater in TSB and R2AB than in BHIB, MHB, or ASH broth. TSB and R2AB were also compared for their recovery efficiency. Generally, there was no significant difference between the numbers of B. cenocepacia grown on 15 differently modified TSA and five modified R2A solid media. Overall, however, diluted TSA and TSB media, and R2A and R2AB showed better recovery efficiency than TSA and TSB for inocula containing small numbers of cells. All strains persisted in distilled water for 40 days. Broth media were more effective than solid media for recovery of B. cenocepacia from distilled water."
According to the news reporters, the research concluded: "These results may assist in improving detection assays with recovery and enrichment strategies to maximize recovery of these fastidious organisms."
For more information on this research see: Evaluation of liquid and solid culture media for the recovery and enrichment of Burkholderia cenocepacia from distilled water. Journal of Industrial Microbiology & Biotechnology, 2014;41(7):1109-1118. Journal of Industrial Microbiology & Biotechnology can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Journal of Industrial Microbiology & Biotechnology - www.springerlink.com/content/1367-5435/)
Our news journalists report that additional information may be obtained by contacting Y. Ahn, Food & Drug Administration, Off Pharmaceut Sci, Center Drug Evaluat & Res, Beltsville, MD, United States. Additional authors for this research include J.M. Kim, H. Ahn, Y.J. Lee, J.J. LiPuma, D. Hussong and C.E. Cerniglia (see also Biotechnology).
Keywords for this news article include: Biotechnology, Maryland, Beltsville, United States, North and Central America
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