Report Summarizes Molecular Therapeutics Study Findings from University of Rochester School of Medicine and Dentistry (Nanoparticle-mediated gene silencing confers radioprotection to salivary glands in vivo)
By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting originating from Rochester, New York, by NewsRx correspondents, research stated, "Radiation treatment of head and neck cancers causes irreversible damage of the salivary glands (SG). Here, we introduce a preclinical mouse model for small-interfering RNA (siRNA)-based gene silencing to provide protection of SG from radiation-induced apoptosis."
Our news editors obtained a quote from the research from the University of Rochester School of Medicine and Dentistry, "Novel, pH-responsive nanoparticles complexed with siRNAs were introduced into mouse submandibular glands (SMG) by retroductal injection to modulate gene expression in vivo. To validate this approach, we first targeted Nkcc1, an ion transporter that is essential for saliva secretion. Nkcc1 siRNA delivery resulted in efficient knockdown, as quantified at the mRNA and the protein levels, and the functional result of Nkcc1 knockdown phenocopied the severe decrease in saliva secretion, characteristic of the systemic Nkcc1 gene knockout. To establish a strategy to prevent apoptotic cell loss due to radiation damage, siRNAs targeting the proapoptotic Pkc? gene were administered into SMG before ionizing radiation. Knockdown of Pkc? not only reduced the number of apoptotic cells during the acute phase of radiation damage, but also markedly improved saliva secretion at 3 months in irradiated animals, indicating that this treatment confers protection from hyposalivation."
According to the news editors, the research concluded: "These results demonstrate that nanoparticle delivery of siRNAs targeting a proapoptotic gene is a localized, nonviral, and effective means of conferring radioprotection to the SGs."
For more information on this research see: Nanoparticle-mediated gene silencing confers radioprotection to salivary glands in vivo. Molecular Therapy, 2013;21(6):1182-94. (Elsevier - www.elsevier.com; Molecular Therapy - www.elsevier.com/wps/product/cws_home/622922)
The news editors report that additional information may be obtained by contacting S. Arany, Center for Oral Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States. Additional authors for this research include D.S. Benoit, S. Dewhurst and C.E Ovitt (see also Biotechnology).
Keywords for this news article include: Biotechnology, New York, Rochester, United States, North and Central America.
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