Peking University Details Findings in Stem Cells (The epigenetic promotion of osteogenic differentiation of human adipose-derived stem cells by the genetic and chemical blockade of histone demethylase LSD1)
By a News Reporter-Staff News Editor at Biotech Week -- A new study on Stem Cell Research is now available. According to news reporting originating in Beijing, People's Republic of China, by NewsRx journalists, research stated, "Human adipose-derived stem cells (hASCs) are a highly attractive source in bone tissue engineering. It has become increasingly clear that chromatin regulators play an important role in cell fate determination."
The news reporters obtained a quote from the research from Peking University, "However, how osteogenic differentiation of hASCs is controlled by epigenetic mechanisms is not fully understood. Here we use genetic tools and chemical inhibitors to modify the epigenetic program of hASCs and identify lysine-specific demethylase 1 (LSD1), a histone demethylase that specifically catalyzes demethylation of di- and mono- methyl histone H3 lysine 4 (H3K4me2/1), as a key regulator in osteogenic differentiation of hASCs. Specifically, we demonstrated that genetic depletion of LSD1 with lentiviral strategy for gene knockdown promoted osteogenic differentiation of hASCs by cell studies and xenograft assays. At the molecular level, we found that LSD1 regulates osteogenesis-associated genes expression through its histone demethylase activity. Significantly, we demonstrated LSD1 demethylase inhibitors could efficiently block its catalytic activity and epigenetically boost osteogenic differentiation of hASCs. Altogether, our study defined the functional and biological roles of LSD1 and extensively explored the effects of its enzymatic activity in osteogenic differentiation of hASCs."
According to the news reporters, the research concluded: "A better understanding of how LSD1 influences on osteogenesis associated epigenetic events will provide new insights into the modulation of hASCs based cell therapy and improve the development of bone tissue engineering with epigenetic intervention."
For more information on this research see: The epigenetic promotion of osteogenic differentiation of human adipose-derived stem cells by the genetic and chemical blockade of histone demethylase LSD1. Biomaterials, 2014;35(23):6015-6025. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
Our news correspondents report that additional information may be obtained by contacting W.S. Ge, Peking University, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, People's Republic of China. Additional authors for this research include Y.S. Liu, T. Chen, X. Zhang, L.W. Lv, C.Y. Jin, Y. Jiang, L. Shi and Y.S. Zhou (see also Stem Cell Research).
Keywords for this news article include: Asia, Tissue Engineering, Biomedical Engineering, Biomedicine, Beijing, Genetics, Bone Research, Bioengineering, Nucleoproteins, Stem Cell Research, Histone Demethylases, Enzymes and Coenzymes, People's Republic of China, N-Demethylating Oxidoreductases
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