New Small Interference RNAs (siRNAs) Findings Reported from Hokkaido University (Gene silencing via RNAi and siRNA quantification in tumor tissue using MEND, a liposomal siRNA delivery system)
By a News Reporter-Staff News Editor at Biotech Week -- A new study on Small Interference RNAs (siRNAs) is now available. According to news reporting originating in Sapporo, Japan, by NewsRx journalists, research stated, "Small interfering RNA (siRNA) would be predicted to function as a cancer drug, but an efficient siRNA delivery system is required for clinical development. To address this issue, we developed a liposomal siRNA carrier, a multifunctional envelope-type nanodevice (MEND)."
The news reporters obtained a quote from the research from Hokkaido University, "We previously reported that a MEND composed of a pH-sensitive cationic lipid, YSK05, showed significant knockdown in both in vitro and in tumor tissue by intratumoral injection. Here, we report on the development of an in vivo siRNA delivery system that is delivered by systemic injection and an analysis of the pharmacokinetics of an intravenously administered siRNA molecule in tumor tissue. Tumor delivery of siRNA was quantified by means of stem-loop primer quantitative reverse transcriptase PCR (qRT-PCR) method. PEGylation of the YSK-MEND results in the increase in the accumulation of siRNA in tumor tissue from 0.0079% ID/g tumor to 1.9% ID/g tumor. The Administration of the MEND (3?mg siRNA/kg body weight) showed about a 50% reduction in the target gene mRNA and protein. Moreover, we verified the induction of RNA interference by 5' RACE-PCR method."
According to the news reporters, the research concluded: "The collective results reported here indicate that an siRNA carrier was developed that can deliver siRNA to a target cell in tumor tissue through an improved siRNA bioavailability."
For more information on this research see: Gene silencing via RNAi and siRNA quantification in tumor tissue using MEND, a liposomal siRNA delivery system. Molecular Therapy, 2013;21(6):1195-203. (Elsevier - www.elsevier.com; Molecular Therapy - www.elsevier.com/wps/product/cws_home/622922)
Our news correspondents report that additional information may be obtained by contacting Y. Sakurai, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan. Additional authors for this research include H. Hatakeyama, Y. Sato, M. Hyodo, H. Akita and H. Harashima (see also Small Interference RNAs (siRNAs)).
Keywords for this news article include: Asia, Japan, Sapporo, Genetics, Liposomes, Small Interference RNAs (siRNAs).
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