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New Metalloendopeptidases Study Findings Have Been Reported by Researchers at University of South Carolina School of Medicine (KLF4 overexpression...

July 16, 2014



New Metalloendopeptidases Study Findings Have Been Reported by Researchers at University of South Carolina School of Medicine (KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases ...)

By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Enzymes and Coenzymes. According to news reporting originating from Columbia, South Carolina, by NewsRx correspondents, research stated, "Neuroblastoma is a childhood tumor that arises from immature neuroblasts of the sympathetic nervous system. Krupple-like factor 4 (KLF4) is a transcription factor, the precise function of which in neuroblastoma is unclear."

Our news editors obtained a quote from the research from the University of South Carolina School of Medicine, "We examined the effects of KLF4 overexpression and apigenin (APG) treatment in human malignant neuroblastoma SK-N-DZ and IMR-32 cell lines. KLF4 overexpression in both SK-N-DZ and IMR-32 cell lines was confirmed by laser scanning immunofluorescent confocal microscopy and Western blotting. We found that 100 nM KLF4 plasmid and 25 ?M APG synergistically inhibited the growth of SK-N-DZ and IMR-32 cells. We also found increase in KLF4 expression in response to treatment with various concentrations of APG. Combination of KLF4 plasmid and APG treatment significantly increased the amounts of apoptosis in both cell lines when compared with control vector or single treatment. We also noticed that the combination therapy decreased expression of the anti-apoptotic proteins Bcl-2 and Mcl-1, increased expression of the pro-apoptotic proteins Bax, Noxa, and Puma, upregulated p53, and caused activation of caspase-3 for cleavage of the inhibitor of caspase-activated DNase (ICAD) leading to completion of apoptosis machinery. Further, combination of KLF4 overexpression and APG treatment was highly effective in inhibiting migration of both neuroblastoma cell lines and was associated with down regulation of matrix metalloproteinases (MMPs) such as MMP-2 and MMP-9."

According to the news editors, the research concluded: "Collectively, our results from this investigation strongly suggest that KLF4 functions as a tumor suppressor and potentiates the anti-cancer activities of APG in two different human malignant neuroblastoma cell lines."

For more information on this research see: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells. Molecular Oncology, 2013;7(3):464-74. (Elsevier - www.elsevier.com; Molecular Oncology - www.elsevier.com/wps/product/cws_home/709916)

The news editors report that additional information may be obtained by contacting N. Mohan, University of South Carolina School of Medicine, Dept. of Pathology, Microbiology and Immunology, Columbia, SC 29209, United States. Additional authors for this research include W. Ai, M. Chakrabarti, N.L. Banik and S.K Ray (see also Enzymes and Coenzymes).

Keywords for this news article include: Caspase, Columbia, Proteomics, United States, South Carolina, Peptide Hydrolases, Enzymes and Coenzymes, Metalloendopeptidases, North and Central America, Matrix Metalloproteinases.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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