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New Luminescent Proteins Data Have Been Reported by Investigators at National Institute of Advanced Industrial Science and Technology (NIAIST)...

July 15, 2014



New Luminescent Proteins Data Have Been Reported by Investigators at National Institute of Advanced Industrial Science and Technology (NIAIST) (Functional artificial luciferases as an optical readout for bioassays)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteins. According to news reporting out of Ibaraki, Japan, by NewsRx editors, research stated, "This study elucidates functional artificial luciferases (ALucs) wholly synthesized for bioassays and molecular imaging. The ALucs bearing epitopes were newly created by amending the sequences of our previously reported ALucs in light of a multi-sequence alignment and hydrophobicity search."

Our news journalists obtained a quote from the research from the National Institute of Advanced Industrial Science and Technology (NIAIST), "The synthesized ALucs are survived in live cells and stable in culture media for 25 days after secretion. The epitopes in ALucs are exposed during the secretion process and indeed valid for column purification and immunological assays. The ALucs exerted a 9400-times stronger optical intensity with a coelenterazine derivative (CTZ i), when compared with Renilla reniformis luciferase 8.6-535. A supersecondary structure of ALuc30 was predicted with respect to the X-ray crystallographic information of the coelenterazine-binding protein (CBP)."

According to the news editors, the research concluded: "The structure revealed that ALuc30 has a room for accommodating the iodide of CTZ i. This study guides on how to create functional artificial luciferases and predicts the structural details with the current bioinformatics technologies."

For more information on this research see: Functional artificial luciferases as an optical readout for bioassays. Biochemical and Biophysical Research Communications, 2014;448(4):418-423. Biochemical and Biophysical Research Communications can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Biochemical and Biophysical Research Communications - www.elsevier.com/wps/product/cws_home/622790)

Our news journalists report that additional information may be obtained by contacting S.B. Kim, Natl Inst Adv Ind Sci & Technol, Res Inst Environm Management Technol, Tsukuba, Ibaraki 3058569, Japan (see also Proteins).

Keywords for this news article include: Asia, Japan, Ibaraki, Luciferases, Luminescent Proteins, Enzymes and Coenzymes

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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