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New Findings in Environmental Microbiology Described from Oak Ridge National Laboratory (Comparative metagenomic and rRNA microbial diversity...

July 14, 2014



New Findings in Environmental Microbiology Described from Oak Ridge National Laboratory (Comparative metagenomic and rRNA microbial diversity characterization using archaeal and bacterial synthetic communities)

By a News Reporter-Staff News Editor at Biotech Business Week -- New research on Environmental Microbiology is the subject of a report. According to news reporting from Oak Ridge, Tennessee, by NewsRx journalists, research stated, "Next-generation sequencing has dramatically changed the landscape of microbial ecology, large-scale and in-depth diversity studies being now widely accessible. However, determining the accuracy of taxonomic and quantitative inferences and comparing results obtained with different approaches are complicated by incongruence of experimental and computational data types and also by lack of knowledge of the true ecological diversity."

The news correspondents obtained a quote from the research from Oak Ridge National Laboratory, "Here we used highly diverse bacterial and archaeal synthetic communities assembled from pure genomic DNAs to compare inferences from metagenomic and SSU rRNA amplicon sequencing. Both Illumina and 454 metagenomic data outperformed amplicon sequencing in quantifying the community composition, but the outcome was dependent on analysis parameters and platform. New approaches in processing and classifying amplicons can reconstruct the taxonomic composition of the community with high reproducibility within primer sets, but all tested primers sets lead to significant taxon-specific biases."

According to the news reporters, the research concluded: "Controlled synthetic communities assembled to broadly mimic the phylogenetic richness in target environments can provide important validation for fine-tuning experimental and computational parameters used to characterize natural communities."

For more information on this research see: Comparative metagenomic and rRNA microbial diversity characterization using archaeal and bacterial synthetic communities. Environmental Microbiology, 2013;15(6):1882-99. (Wiley-Blackwell - www.wiley.com/; Environmental Microbiology - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920)

Our news journalists report that additional information may be obtained by contacting M. Shakya, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 47841, United States. Additional authors for this research include C. Quince, J.H. Campbell, Z.K. Yang, C.W. Schadt and M. Podar (see also Environmental Microbiology).

Keywords for this news article include: Oak Ridge, Tennessee, United States, North and Central America, Environmental Microbiology.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Business Week


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