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New Data from Guangzhou Medical University Illuminate Findings in Biomedicine and Biomedical Engineering (Simultaneous stereoselective analysis of...

July 16, 2014



New Data from Guangzhou Medical University Illuminate Findings in Biomedicine and Biomedical Engineering (Simultaneous stereoselective analysis of naftopidil and O-desmethyl naftopidil enantiomers in rat feces using an online column-switching ...)

By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Biotechnology have been presented. According to news reporting from Guangdong, People's Republic of China, by NewsRx journalists, research stated, "A novel online column-switching chiral high-performance liquid chromatography method was developed and validated for the simultaneous determination of naftopidil (NAF) and its O-desmethyl metabolites (DMN) enantiomers in rat feces. Direct and multiple injections of supernatant from rat feces homogenate were allowed through the column-switching system."

The news correspondents obtained a quote from the research from Guangzhou Medical University, "Analyte extraction was performed on the Capcell Pak mixed-functional column by acetonitrile-phosphate buffer (pH7.4; 10mm; 8:92, v/v) flowing at 1mL/min. Separation of NAF and DMN enantiomers was achieved on the Chiralpak IA column by methanol-acetonitrile-acetate buffer (pH5.3; 5mm; 45:33:22, v/v/v) flowing at 0.5mL/min. The analytes were measured with a fluorescence detector at 290nm (lambda(ex)) and 340nm (lambda(em)). The validated method showed a good linearity [22.5-15,000ng/mL for (+)-/(-)-NAF; 35-25,000ng/mL for (+)-/(-)-DMN] and the lowest limits of quantification for NAF and DMN enantiomers were 22.5 and 35ng/mL, respectively. Both intra- and inter-day variations were

According to the news reporters, the research concluded: "Nonstereoselective excretion of (+)- and (-)-NAF was found in feces, while stereoselective excretion of (+)- and (-)-DMN was observed with higher excretion levels of (+)-DMN, indicating that there may exist stereoselective metabolism for NAF enantiomers."

For more information on this research see: Simultaneous stereoselective analysis of naftopidil and O-desmethyl naftopidil enantiomers in rat feces using an online column-switching high-performance liquid chromatography method. Biomedical Chromatography, 2014;28(7):1030-1035. Biomedical Chromatography can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Biomedical Chromatography - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-0801)

Our news journalists report that additional information may be obtained by contacting Y.Y. Zhang, Guangzhou Med Univ, Pharmaceut Res Center, Guangzhou 510182, Guangdong, People's Republic of China. Additional authors for this research include X.W. Liu, L.J. Zhu and M. Yuan (see also Biotechnology).

Keywords for this news article include: Asia, Biotechnology, Guangdong, Imaging Technology, People's Republic of China, High-Performance Liquid Chromatography

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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