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New Biotechnology and Bioengineering Study Findings Have Been Reported by Researchers at Vanderbilt University (ETA: robust software for...

July 16, 2014



New Biotechnology and Bioengineering Study Findings Have Been Reported by Researchers at Vanderbilt University (ETA: robust software for determination of cell specific rates from extracellular time courses)

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Biotechnology have been published. According to news reporting out of Nashville, Tennessee, by NewsRx editors, research stated, "Accurate quantification of cell specific rates and their uncertainties is of critical importance for assessing metabolic phenotypes of cultured cells. We applied two different methods of regression and error analysis to estimate specific metabolic rates from time-course measurements obtained in exponentially growing cell cultures."

Our news journalists obtained a quote from the research from Vanderbilt University, "Using simulated data sets to compute specific rates of growth, glucose uptake, and lactate excretion, we found that Gaussian error propagation from prime variables to the final calculated rates was the most accurate method for estimating parameter uncertainty. We incorporated this method into a MATLAB-based software package called Extracellular Time-Course Analysis (ETA), which automates the analysis workflow required to (i) compute cell specific metabolic rates and their uncertainties; (ii) test the goodness-of-fit of the experimental data to the regression model; and (iii) rapidly compare the results across multiple experiments. ETA was used to estimate the uptake or excretion rate of glucose, lactate, and 18 different amino acids in a B-cell model of c-Myc-driven cancer. We found that P493-6 cells with High Myc expression increased their specific uptake of glutamine, arginine, serine, lysine, and branched-chain amino acids by two-to threefold in comparison to low Myc cells, but exhibited only modest increases in glucose uptake and lactate excretion."

According to the news editors, the research concluded: "By making the ETA software package freely available to the scientific community, we expect that it will become an important tool for rigorous estimation of specific rates required for metabolic flux analysis and other quantitative metabolic studies."

For more information on this research see: ETA: robust software for determination of cell specific rates from extracellular time courses. Biotechnology & Bioengineering, 2013;110(6):1748-58. (Wiley-Blackwell - www.wiley.com/; Biotechnology & Bioengineering - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290)

Our news journalists report that additional information may be obtained by contacting T.A. Murphy, Dept. of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37235, United States (see also Biotechnology).

Keywords for this news article include: Biotechnology, Software, Nashville, Tennessee, United States, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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