Investigators from University of Ege Target Cancer Gene Therapy (Notch Signaling-related Therapeutic Strategies With Novel Drugs in Neuroblastoma Spheroids)
By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators publish new report on Biotechnology. According to news reporting originating from Izmir, Turkey, by NewsRx correspondents, research stated, "Neuroblastoma is a severe pediatric tumor characterized by poor prognosis. Identification of novel molecular targets and diversion of investigations on new drug trials is mandatory for cancer therapy."
Our news editors obtained a quote from the research from the University of Ege, "In this study, vinorelbine tartrate, lithium chloride, clomipramine, and medroxyprogesterone acetate are used for the possible new treatment modalities in neuroblastoma cells. Notch and c-kit are novel molecules in cancer research, and Notch pathway is one of the emerging molecules in the neuroblastoma pathogenesis. Cytotoxic effects of these drugs at different time points, with different doses were studied in the SH-SY5Y human neuroblastoma cell line. Analysis of Notch and c-kit signaling with immunohistochemistry were constituted in multicellular tumor spheroids, and morphologic investigation was performed for digital imaging of cancer stem cells (CSCs) with electron microscopy. Size kinetics of spheroids was also determined after drug treatment. Results showed that all drugs were cytotoxic for neuroblastoma cells. Yet, this cytotoxic action did not correlate with the inhibitory effects in cell signaling. Neuroblastoma spheroids showed increased immunoreactivity of Notch signaling and c-kit. Altered ultrastructural CSCs morphology was observed after clomipramine and medroxyprogesterone acetate treatment compared with other drugs. Lithium chloride showed cellular membrane destruction for both CSCs and the remaining population. In this study, independent effects of cytotoxicity in tumor cells with respect to CSCs were determined."
According to the news editors, the research concluded: "Redundant cells, which are the bulk population in tumor a compound, destroyed with therapy, were neither a target for treatment nor a remarkable investigation of cancer."
For more information on this research see: Notch Signaling-related Therapeutic Strategies With Novel Drugs in Neuroblastoma Spheroids. Journal of Pediatric Hematology Oncology, 2014;36(1):37-44. Journal of Pediatric Hematology Oncology can be contacted at: Lippincott Williams & Wilkins, 530 Walnut St, Philadelphia, PA 19106-3621, USA (see also Biotechnology).
The news editors report that additional information may be obtained by contacting S. Ayla, Ege University, Fac Med, Dept. of Histol & Embryol, TR-35100 Izmir, Turkey. Additional authors for this research include A. Bilir, B.C. Soner, O. Yilmaz-Dilsiz, M. Erguven and G. Oktem.
Keywords for this news article include: Biotechnology, Izmir, Turkey, Eurasia, Oncology, Chemicals, Chemistry, Hematology, Therapeutics, Neuroblastoma, Lithium Chloride, Cancer Gene Therapy
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