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Findings from X. Du and Co-Authors Update Knowledge of Neuroepithelial Cells (Regeneration of mammalian cochlear and vestibular hair cells through...

July 18, 2014



Findings from X. Du and Co-Authors Update Knowledge of Neuroepithelial Cells (Regeneration of mammalian cochlear and vestibular hair cells through Hes1/Hes5 modulation with siRNA)

By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in Sensory Receptor Cells. According to news reporting out of Oklahoma City, Oklahoma, by NewsRx editors, research stated, "The Notch pathway is a cell signaling pathway determining initial specification and subsequent cell fate in the inner ear. Previous studies have suggested that new hair cells (HCs) can be regenerated in the inner ear by manipulating the Notch pathway."

Our news journalists obtained a quote from the research, "In the present study, delivery of siRNA to Hes1 and Hes5 using a transfection reagent or siRNA to Hes1 encapsulated within poly(lactide-co-glycolide acid) (PLGA) nanoparticles increased HC numbers in non-toxin treated organotypic cultures of cochleae and maculae of postnatal day 3 mouse pups. An increase in HCs was also observed in cultured cochleae and maculae of mouse pups pre-conditioned with a HC toxin (4-hydroxy-2-nonenal or neomycin) and then treated with the various siRNA formulations. Treating cochleae with siRNA to Hes1 associated with a transfection reagent or siRNA to Hes1 delivered by PLGA nanoparticles decreased Hes1 mRNA and up-regulated Atoh1 mRNA expression allowing supporting cells (SCs) to acquire a HC fate. Experiments using cochleae and maculae of p27(kip1)/-GFP transgenic mouse pups demonstrated that newly generated HCs trans-differentiated from SCs. Furthermore, PLGA nanoparticles are non-toxic to inner ear tissue, readily taken up by cells within the tissue of interest, and present a synthetic delivery system that is a safe alternative to viral vectors."

According to the news editors, the research concluded: "These results indicate that when delivered using a suitable vehicle, Hes siRNAs are potential therapeutic molecules that may have the capacity to regenerate new HCs in the inner ear and possibly restore human hearing and balance function."

For more information on this research see: Regeneration of mammalian cochlear and vestibular hair cells through Hes1/Hes5 modulation with siRNA. Hearing Research, 2013;304():91-110. (Elsevier - www.elsevier.com; Hearing Research - www.elsevier.com/wps/product/cws_home/506060)

Our news journalists report that additional information may be obtained by contacting X. Du, Hough Ear Institute, PO Box 23206, Oklahoma City, OK 73112, United States. Additional authors for this research include W. Li, X. Gao, M.B. West, W.M. Saltzman, C.J. Cheng, C. Stewart, J. Zheng, W. Cheng and R.D Kopke (see also Sensory Receptor Cells).

Keywords for this news article include: Ear Research, Nanoparticle, Oklahoma City, United States, Nanotechnology, Acoustic Maculae, Afferent Neurons, Emerging Technologies, Neuroepithelial Cells, Vestibular Hair Cells, Sensory Receptor Cells, North and Central America, Peripheral Nervous System.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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