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Findings from Wuhan University Broadens Understanding of Capillary Electrophoresis (Membrane-supported liquid-liquid-liquid microextraction combined...

July 15, 2014



Findings from Wuhan University Broadens Understanding of Capillary Electrophoresis (Membrane-supported liquid-liquid-liquid microextraction combined with anion-selective exhaustive injection capillary electrophoresis-ultraviolet detection for ...)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Capillary Electrophoresis. According to news reporting originating in Wuhan, People's Republic of China, by NewsRx journalists, research stated, "A novel method based on off-line membrane-supported liquid-liquid-liquid microextraction (MS-LLLME) combined with on-column anion-selective exhaustive injection (ASEI) capillary electrophoresis-ultraviolet (CE-UV) detection was established for the analysis of seven phytohormones (abscisic acid (ABA), jasmonic acid (JA), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), salicylic acid (SA) and gibberellic acid (GA)). In MS-LLLME, the target phytohormones were extracted from the acid donor phase to the alkaline acceptor phase, and the acceptor solutions were directly analyzed by ASEI-CE-UV."

The news reporters obtained a quote from the research from Wuhan University, "Under the optimal experimental conditions, the analytical performance of the method was evaluated. The limits of detection (LODs) of ABA, JA, 2,4-D, NAA, IAA, SA and GA were determined to be 1.00, 2.21, 0.33, 0.17, 0.67, 0.05 and 16.5ng/mL, respectively. The relative standard deviations (RSDs, n=7) ranged from 4.7% to 12.9%, and the enrichment factors were in the range of 307 to 20,160. The proposed method was successfully applied for the determination of multiple phytohormones in banana, cabbage and cucumber extracts, and ABA, IAA and SA were detected in these samples. The recoveries for the spiked samples were in the range of 79.0 to 116.4%."

According to the news reporters, the research concluded: "The proposed method was demonstrated to be suitable for the simultaneous quantification of multiple phytohormones with high sensitivity and good sample cleanup ability."

For more information on this research see: Membrane-supported liquid-liquid-liquid microextraction combined with anion-selective exhaustive injection capillary electrophoresis-ultraviolet detection for sensitive analysis of phytohormones. Journal of Chromatography A, 2014;1343():10-7. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)

Our news correspondents report that additional information may be obtained by contacting L. Huang, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Dept. of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China. Additional authors for this research include M. He, B. Chen and B. Hu (see also Capillary Electrophoresis).

Keywords for this news article include: Asia, Wuhan, Capillary Electrophoresis, People's Republic of China.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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