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Findings from Shanghai Jiao-Tong University in Molecular Pharmacology Reported (Characterization of the Zebrafish Ugt Repertoire Reveals a New Class...

July 16, 2014

Findings from Shanghai Jiao-Tong University in Molecular Pharmacology Reported (Characterization of the Zebrafish Ugt Repertoire Reveals a New Class of Drug-Metabolizing UDP Glucuronosyltransferases)

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "The zebrafish genome contains a gene superfamily of 40 Ugt genes that can be divided into Ugt1, Ugt2, and Ugt5 families. Because the encoded zebrafish UDP glucuronosyltransferase (UGT) proteins do not display orthologous relationships to any of the mammalian and avian UGT enzymes based on molecular phylogeny, it is difficult to predict their substrate specificity."

Our news journalists obtained a quote from the research from Shanghai Jiao-Tong University, "Here, we mapped their tissue-specific expression patterns. We showed that the zebrafish UGT enzymes can be glycosylated. We determined their substrate specificity and catalytic activity toward diverse aglycone substrates. Specifically, we measured mRNA levels of each of the 40 zebrafish Ugt genes in 11 adult tissues and found that they are expressed in a tissue-specific manner. Moreover, functional analyses with the donor of UDP glucuronic acid (UDPGA) for each of the 40 zebrafish UGT proteins revealed their substrate specificity toward 10 important aglycones. In particular, UGT1A1, UGT1A7, and UGT1B1 displayed good glucuronidation activities toward most phenolic aglycones (4-methylumbelliferone, 4-nitrophenol, 1-naphthol, bisphenol A, and mycophenolic acid) and the two carboxylic acids (bilirubin and diclofenac). Importantly, some members of the UGT5, a novel UGT family identified recently, are capable of glucuronidating multiple aglycones with the donor cofactor of UDPGA. In particular, UGT5A5, UGT5B2, and UGT5E1 glucuronidate phenols and steroids with high specificity toward steroid hormones of estradiol and testosterone and estrogenic alkylphenols 4-tert-octylphenol."

According to the news editors, the research concluded: "These results shed new insights into the mechanisms by which fish species defend themselves against vast numbers of xenobiotics via glucuronidation conjugations and may facilitate the establishment of zebrafish as a model vertebrate in toxicological, developmental, and pathologic studies."

For more information on this research see: Characterization of the Zebrafish Ugt Repertoire Reveals a New Class of Drug-Metabolizing UDP Glucuronosyltransferases. Molecular Pharmacology, 2014;86(1):62-75. Molecular Pharmacology can be contacted at: Amer Soc Pharmacology Experimental Therapeutics, 9650 Rockville Pike, Bethesda, MD 20814-3995, USA (see also Biotechnology).

The news correspondents report that additional information may be obtained from Y.M. Wang, Shanghai Jiao Tong University, Sch Life Sci & Biotechnol, Bio X Center, Key Lab Genet Dev & Neuropsychiat DisordersMinis, Shanghai 200240, People's Republic of China. Additional authors for this research include H.Y. Huang and Q. Wu.

Keywords for this news article include: Asia, Biotechnology, Shanghai, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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