Findings from Rensselaer Polytechnic Institute in the Area of DNA Research Reported (Sequence-based separation of single-stranded DNA using nucleotides in capillary electrophoresis: focus on phosphate)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on DNA Research is now available. According to news reporting from Troy, New York, by NewsRx journalists, research stated, "DNA analysis has widespread applicability in biology, medicine, biotechnology, and forensics. DNA separation by length is readily achieved using sieving gels in electrophoresis."
The news correspondents obtained a quote from the research from Rensselaer Polytechnic Institute, "Separation by sequence is less simple, generally requiring adequate differences in native or induced conformation or differences in thermal or chemical stability of the strands that are hybridized prior to measurement. We previously demonstrated separation of four single-stranded DNA 76-mers that differ by only a few A-G substitutions based solely on sequence using guanosine-5'-monophosphate (GMP) in the running buffer. We attributed separation to the unique self-assembly of GMP to form higher order structures. Here, we examine an expanded set of 76-mers designed to probe the mechanism of the separation and effects of experimental conditions. We were surprised to find that other ribonucleotides achieved the similar separation to GMP, and that some separation was achieved using sodium phosphate instead of GMP. Potassium phosphate achieved almost as good separations as the ribonucleotides. This suggests that the separation medium provides a physicochemical environment for the DNA that effects strand migration in a sequence-selective manner. Further investigation is needed to determine whether the mechanism involves specific interactions between the phosphates and the DNA strands or is a result of other properties of the separation medium. Phosphate generally has been avoided in DNA separations by capillary gel electrophoresis because its high ionic strength exacerbates Joule heating."
According to the news reporters, the research concluded: "Our results suggest that phosphate compounds should be examined for separation of DNA based on sequence."
For more information on this research see: Sequence-based separation of single-stranded DNA using nucleotides in capillary electrophoresis: focus on phosphate. Electrophoresis, 2013;34(12):1778-86. (Wiley-Blackwell - www.wiley.com/; Electrophoresis - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1522-2683)
Our news journalists report that additional information may be obtained by contacting X. Zhang, Dept. of Chemistry and Chemical Biology, 321 Cogswell Laboratory, Rensselaer Polytechnic Institute, Troy, NY 12180, United States (see also DNA Research).
Keywords for this news article include: Troy, Anions, New York, Phosphates, DNA Research, United States, Phosphoric Acids, North and Central America, Capillary Electrophoresis.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC