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Findings from Los Alamos National Laboratory Provides New Data on Cysts (Functionalization-dependent induction of cellular survival pathways by CdSe...

July 18, 2014



Findings from Los Alamos National Laboratory Provides New Data on Cysts (Functionalization-dependent induction of cellular survival pathways by CdSe quantum dots in primary normal human bronchial epithelial cells)

By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Research findings on Cysts are discussed in a new report. According to news reporting originating in Los Alamos, New Mexico, by NewsRx journalists, research stated, "Quantum dots (QDs) are semiconductor nanocrystals exhibiting unique optical properties that can be exploited for many practical applications ranging from photovoltaics to biomedical imaging and drug delivery. A significant number of studies have alluded to the cytotoxic potential of these materials, implicating Cd-leaching as the causal factor."

The news reporters obtained a quote from the research from Los Alamos National Laboratory, "Here, we investigated the role of heavy metals in biological responses and the potential of CdSe-induced genotoxicity. Our results indicate that, while negatively charged QDs are relatively noncytotoxic compared to positively charged QDs, the same does not hold true for their genotoxic potential. Keeping QD core composition and size constant, 3 nm CdSe QD cores were functionalized with mercaptopropionic acid (MPA) or cysteamine (CYST), resulting in negatively or positively charged surfaces, respectively. CYST-QDs were found to induce significant cytotoxicity accompanied by DNA strand breakage. However, MPA-QDs, even in the absence of cytotoxicity and reactive oxygen species formation, also induced a high number of DNA strand breaks. QD-induced DNA damage was confirmed by identifying the presence of p53 binding protein 1 (53BP1) in the nuclei of exposed cells and subsequent diminishment of p53 from cytoplasmic cellular extracts. Further, high-throughput real-time PCR analyses revealed upregulation of DNA damage and response genes and several proinflammatory cytokine genes. Most importantly, transcriptome sequencing revealed upregulation of the metallothionein family of genes in cells exposed to MPA-QDs but not CYST-QDs."

According to the news reporters, the research concluded: "These data indicate that cytotoxic assays must be supplemented with genotoxic analyses to better understand cellular responses and the full impact of nanoparticle exposure when making recommendations with regard to risk assessment."

For more information on this research see: Functionalization-dependent induction of cellular survival pathways by CdSe quantum dots in primary normal human bronchial epithelial cells. Acs Nano, 2013;7(10):8397-411. (American Chemical Society - www.acs.org; Acs Nano - www.pubs.acs.org/journal/ancac3)

Our news correspondents report that additional information may be obtained by contacting A. Nagy, Bioscience Division, Center for Integrated Nanotechnologies, Materials Physics & Applications Division and Chemical Diagnostics and Engineering, Los Alamos National Laboratory , Los Alamos, New Mexico 87545, United States. Additional authors for this research include J.A. Hollingsworth, B. Hu, A. Steinbruck, P.C. Stark, C. Rios Valdez, M. Vuyisich, M.H. Stewart, D.H. Atha, B.C. Nelson and R. Iyer (see also Cysts).

Keywords for this news article include: Cysts, Genetics, p53 Gene, Los Alamos, New Mexico, Quantum Dots, United States, Nanotechnology, Quantum Physics, Epithelial Cells, Emerging Technologies, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Genomics & Genetics Weekly


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