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Emory University School of Medicine Reports Findings in Stem Cells (Induction of pluripotency in bone marrow mononuclear cells via polyketal...

July 14, 2014



Emory University School of Medicine Reports Findings in Stem Cells (Induction of pluripotency in bone marrow mononuclear cells via polyketal nanoparticle-mediated delivery of mature microRNAs)

By a News Reporter-Staff News Editor at Hematology Week -- New research on Stem Cell Research is the subject of a report. According to news originating from Atlanta, Georgia, by NewsRx correspondents, research stated, "Since the successful generation of induced pluripotent stem cells (iPSC) from adult somatic cells using integrating-viral methods, various methods have been tried for iPSC generation using non-viral and non-integrating technique for clinical applications. Recently, various non-viral approaches such as protein, mRNA, microRNA, and small molecule transduction were developed to avoid genomic integration and generate stem cell-like cells from mouse and human fibroblasts."

Our news journalists obtained a quote from the research from the Emory University School of Medicine, "Despite these successes, there has been no successful generation of iPSC from bone marrow (BM)-derived hematopoietic cells derived using non-viral methods to date. Previous reports demonstrate the ability of polymeric micro and nanoparticles made from polyketals to deliver various molecules to macrophages. MicroRNA-loaded nanoparticles were created using the polyketal polymer PK3 (PK3-miR) and delivered to somatic cells for 6 days, resulting in the formation of colonies. Isolated cells from these colonies were assayed and substantial induction of the pluripotency markers Oct4, Sox2, and Nanog were detected. Moreover, colonies transferred to feeder layers also stained positive for pluripotency markers including SSEA-1. Here, we demonstrate successful activation of pluripotency-associated genes in mouse BM-mononuclear cells using embryonic stem cell (ESC)-specific microRNAs encapsulated in the acid sensitive polyketal PK3."

According to the news editors, the research concluded: "These reprogramming results demonstrate that a polyketal-microRNA delivery vehicle can be used to generate various reprogrammed cells without permanent genetic manipulation in an efficient manner."

For more information on this research see: Induction of pluripotency in bone marrow mononuclear cells via polyketal nanoparticle-mediated delivery of mature microRNAs. Biomaterials, 2013;34(17):4235-41. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)

The news correspondents report that additional information may be obtained from Y.D. Sohn, Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322, United States. Additional authors for this research include I. Somasuntharam, P.L. Che, R. Jayswal, N. Murthy, M.E. Davis and Y.S Yoon (see also Stem Cell Research).

Keywords for this news article include: Viral, Virus, Atlanta, Georgia, Bone Marrow, Nanoparticle, United States, Bone Research, Immune System, Nanotechnology, Stem Cell Research, Emerging Technologies, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Hematology Week


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