Data on Liposomes Reported by Researchers at North Dakota State University (Influence of short-chain cell-penetrating peptides on transport of Doxorubicin encapsulating receptor-targeted liposomes across brain endothelial barrier)
By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Biotechnology. According to news originating from Fargo, North Dakota, by NewsRx correspondents, research stated, "To investigate the influence of different cell penetrating peptides (CPPs-TAT, Penetratin and Mastoparan), on the transport of doxorubicin encapsulating transferrin (Tf)-liposomes across brain endothelial barrier, in vitro and in vivo. The cellular uptake of dual-functionalized, (Tf-CPP), liposomes into various tumor cells was assessed using HPLC."
Our news journalists obtained a quote from the research from North Dakota State University, "The transport of liposomes was also measured across a robust 3D brain tumor model constructed using chitosan-PLGA scaffolds. The growth of tumor cells was monitored using H&E staining and the fully grown tumor scaffolds were visualized using SEM. The tumor scaffolds were combined with the culture inserts carrying tightly packed brain endothelial cells. The in vitro and in vivo transport of drug (using Tf-CPP-liposomes) across the brain endothelial barrier was determined by extraction of the drug from cells and tissues followed by analysis using HPLC. The results demonstrated improved delivery of doxorubicin using dual-functionalized liposomes versus the single ligand or unmodified liposomes. Among different Tf-CPP-liposomes, the Tf-Penetratin liposomes showed efficient cellular transport of the encapsulated drug (approximately 90-98%) and maximum translocation of the drug across the brain endothelial barrier (approximately 15% across in vitro and 4% across in vivo BBB). The Tf-Penetratin and Tf-TAT liposomes demonstrated excellent cellular biocompatibility and no hemolytic activity upto 200nM phospholipid concentration. The Tf-CPP liposomes showed efficient translocation of the anticancer drug across the brain endothelial barrier."
According to the news editors, the research concluded: "In addition, an absolute and robust in vitro brain tumor model was successfully constructed to overcome the practical intricacies of developing a successful in vivo orthotopic brain tumor model."
For more information on this research see: Influence of short-chain cell-penetrating peptides on transport of Doxorubicin encapsulating receptor-targeted liposomes across brain endothelial barrier. Pharmaceutical Research, 2014;31(5):1194-209. Pharmaceutical Research can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Pharmaceutical Research - www.springerlink.com/content/0724-8741/)
The news correspondents report that additional information may be obtained from G. Sharma, Dept. of Pharmaceutical Sciences College of Pharmacy Nursing and Allied Sciences, North Dakota State University, Fargo, North Dakota, 58108, United States. Additional authors for this research include A. Modgil, T. Zhong, C. Sun and J. Singh (see also Biotechnology).
The publisher's contact information for the journal Pharmaceutical Research is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Fargo, North Dakota, United States, North and Central America, Antibiotics Antineoplastics, Bioengineering, Biomedical Engineering, Biomedicine, Biotechnology, Doxorubicin Hydrochloride, Drug Delivery Systems, Drugs, Liposomes, Pharmaceuticals, Therapy, Tissue Engineering.
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