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Study Results from University of Fortaleza Broaden Understanding of Reproduction and Fertility [Protein profile of the seminal plasma of collared...

August 4, 2014



Study Results from University of Fortaleza Broaden Understanding of Reproduction and Fertility [Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)]

By a News Reporter-Staff News Editor at Biotech Business Week -- Current study results on Life Science Research have been published. According to news reporting originating in Fortaleza, Brazil, by NewsRx journalists, research stated, "This study was conducted to characterize the major proteins of the peccary seminal plasma, based on the semen samples collected from nine adult and reproductively sound animals. Our approach included the use of two-dimensional electrophoresis followed by Coomassie blue staining and analysis of polypeptide maps with PDQuest Software (Bio-Rad)."

The news reporters obtained a quote from the research from the University of Fortaleza, "Proteins were identified by tandem mass spectrometry (LC-MS/MS). We detected 179 protein spots per gel and 98 spots were identified by mass spectrometry, corresponding to 23 different proteins. The combined intensity of those spots accounted for 56.2 +/- 6% of the intensities of all spots and 60.9% of the intensities of spots presented in every protein map. Protein spots identified as clusterin represented 19.7 +/- 8.3% of the integrated optical densities of all spots detected in the seminal plasma maps. There was a negative association (r=-0.87; P< 0.05) between the intensity of a clusterin spot and the percentage of sperm with functional membrane. Spermadhesin porcine seminal plasma protein 1 and bodhesin 2 comprised 5.4 +/- 1.9 and 8.8 +/- 3.9% of the total intensity of all spots respectively. Many proteins appeared in a polymorphic pattern, such as clusterin (27 spots), epididymal secretory glutathione peroxidase (ten spots), inter-alpha-trypsin inhibitor (12 spots), and IgG-binding protein (ten spots), among others."

According to the news reporters, the research concluded: "We presently describe the major seminal plasma proteome of the peccary, which exhibits a distinct high expression of clusterin isoforms. Knowledge of wild species reproductive biology is crucial for an understanding of their survival strategies and adaptation in a changing environment."

For more information on this research see: Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758). Reproduction, 2014;147(6):753-764. Reproduction can be contacted at: Bioscientifica Ltd, Euro House, 22 Apex Court Woodlands, Bradley Stoke, Bristol BS32 4JT, England. (Wiley-Blackwell - www.wiley.com/; Reproduction - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0531)

Our news correspondents report that additional information may be obtained by contacting E.A.A. Santos, Univ Fortaleza, Sch Pharm, BR-60811905 Fortaleza, Ceara, Brazil. Additional authors for this research include P.C. Sousa, J.A.M. Martins, R.A. Moreira, A.C.O. Monteiro-Moreira, F. Moreno, M.F. Oliveira, A.A. Moura and A.R. Silva (see also Life Science Research).

Keywords for this news article include: Brazil, Fortaleza, South America, Life Science Research

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Business Week


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