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Study Results from Max-Planck-Institute for Colloids and Interfaces in the Area of Intracellular Space Reported (Comparison of Gold Nanoparticle...

August 5, 2014



Study Results from Max-Planck-Institute for Colloids and Interfaces in the Area of Intracellular Space Reported (Comparison of Gold Nanoparticle Mediated Photoporation: Vapor Nanobubbles Outperform Direct Heating for Delivering Macromolecules ...)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Intracellular Space. According to news reporting originating from Potsdam, Germany, by NewsRx correspondents, research stated, "There is a great interest in delivering macromolecular agents into living cells for therapeutic purposes, such as siRNA for gene silencing. Although substantial effort has gone into designing nonviral nanocarriers for delivering macromolecules into cells, translocation of the therapeutic molecules from the endosomes after endocytosis into the cytoplasm remains a major bottleneck."

Our news editors obtained a quote from the research from Max-Planck-Institute for Colloids and Interfaces, "Laser-induced photoporation, especially in combination with gold nanoparticles, is an alternative physical method that is receiving increasing attention for delivering macromolecules in cells. By allowing gold nanoparticles to bind to the cell membrane, nanosized membrane pores can be created upon pulsed laser illumination. Depending on the laser energy, pores are created through either direct heating of the AuNPs or by vapor nanobubbles (VNBs) that can emerge around the AuNPs. Macromolecules in the surrounding cell medium can then diffuse through the pores directly into the cytoplasm. Here we present a systematic evaluation of both photoporation mechanisms in terms of cytotoxicity, cell loading, and siRNA transfection efficiency. We find that the delivery of macromolecules under conditions of VNBs is much more efficient than direct photothermal disturbance of the plasma membrane without any noticeable cytotoxic effect. Interestingly, by tuning the laser energy, the pore size could be changed, allowing control of the amount and size of molecules that are delivered in the cytoplasm."

According to the news editors, the research concluded: "As only a single nanosecond laser pulse is required, we conclude that VNBs are an interesting photoporation mechanism that may prove very useful for efficient high-throughput macromolecular delivery in live cells."

For more information on this research see: Comparison of Gold Nanoparticle Mediated Photoporation: Vapor Nanobubbles Outperform Direct Heating for Delivering Macromolecules in Live Cells. ACS Nano, 2014;8(6):6288-6296. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)

The news editors report that additional information may be obtained by contacting R.H. Xiong, Max Planck Inst Colloids & Interfaces, D-14424 Potsdam, Germany. Additional authors for this research include K. Raemdonck, K. Peynshaert, I. Lentacker, I. De Cock, J. Demeester, S.C. De Smedt, A.G. Skirtach and K. Braeckmans (see also Intracellular Space).

Keywords for this news article include: Europe, Potsdam, Germany, Cytoplasm, Nanobubbles, Nanotechnology, Gold Nanoparticles, Intracellular Space, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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