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Researchers from Yangzhou University Discuss Findings in Nanoparticles (Fluorescence energy transfer-based multiplexed hybridization assay using gold...

August 5, 2014



Researchers from Yangzhou University Discuss Findings in Nanoparticles (Fluorescence energy transfer-based multiplexed hybridization assay using gold nanoparticles and quantum dot conjugates on photonic crystal beads)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Nanoparticles. According to news reporting from Yangzhou, People's Republic of China, by NewsRx journalists, research stated, "A multiplexed assay strategy was developed for the detection of nucleic acid hybridization. It is based on fluorescence resonance energy transfer (FRET) between gold nanoparticles (AuNPs) and multi-sized quantum dots (QDs) deposited on the surface of silica photonic crystal beads (SPCBs)."

The news correspondents obtained a quote from the research from Yangzhou University, "The SPCBs were first coated with a three-layer primer film formed by the alternating adsorption of poly(allylamine hydrochloride) and poly(sodium 4-styrensulfonate). Probe DNA sequences were then covalently attached to the carboxy groups at the surface of the QD-coated SPCBs. On addition of DNA-AuNPs and hybridization, the fluorescence of the donor QDs is quenched because of the close proximity of the AuNPs. However, the addition of target DNA causes a recovery of the fluorescence of the QD-coated SPCBs, thus enabling the quantitative assay of hybridized DNA. Compared to fluorescent dyes acting as acceptors, the use of AuNPs results in much higher quenching efficiency. The multiplexed assay displays a wide linear range, high sensitivity, and very little cross-reactivity."

According to the news reporters, the research concluded: "This work, where such SPCBs are used for the first time in a FRET assay, is deemed to present a new and viable approach towards high-throughput multiplexed gene assays."

For more information on this research see: Fluorescence energy transfer-based multiplexed hybridization assay using gold nanoparticles and quantum dot conjugates on photonic crystal beads. Microchimica Acta, 2014;181(9-10):1109-1115. Microchimica Acta can be contacted at: Springer Wien, Sachsenplatz 4-6, PO Box 89, A-1201 Wien, Austria. (Springer - www.springer.com; Microchimica Acta - www.springerlink.com/content/0026-3672/)

Our news journalists report that additional information may be obtained by contacting J. Li, Yangzhou University, Coll Chem & Chem Engn, Yangzhou 225002, People's Republic of China. Additional authors for this research include H.M. Qi, H. Wang, Z.J. Yang, P.Z. Zhu and G.W. Diao (see also Nanoparticles).

Keywords for this news article include: Asia, Yangzhou, Quantum Dots, Nanotechnology, Emerging Technologies, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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