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Researchers from University of California Detail New Studies and Findings in the Area of Viral RNA (Nanopore-Based Conformational Analysis of a Viral...

August 5, 2014

Researchers from University of California Detail New Studies and Findings in the Area of Viral RNA (Nanopore-Based Conformational Analysis of a Viral RNA Drug Target)

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Viral RNA. According to news reporting originating from La Jolla, California, by NewsRx correspondents, research stated, "Nanopores are single-molecule sensors that show exceptional promise as a biomolecular analysis tool by enabling label-free detection of small amounts of sample. In this paper, we demonstrate that nanopores are capable of detecting the conformation of an antiviral RNA drug target."

Our news editors obtained a quote from the research from the University of California, "The hepatitis C virus uses an internal ribosome entry site (IRES) motif in order to initiate translation by docking to ribosomes in its host cell. The IRES is therefore a viable and important drug target. Drug-induced changes to the conformation of the HCV IRES motif, from a bent to a straight conformation, have been shown to inhibit HCV replication. However, there is presently no straightforward method to analyze the effect of candidate small-molecule drugs on the RNA conformation. In this paper, we show that RNA translocation dynamics through a 3 nm diameter nanopore is conformation-sensitive by demonstrating a difference in transport times between bent and straight conformations of a short viral RNA motif. Detection is possible because bent RNA is stalled in the 3 nm pore, resulting in longer molecular dwell times than straight RNA. Control experiments show that binding of a weaker drug does not produce a conformational change, as consistent with independent fluorescence measurements."

According to the news editors, the research concluded: "Nanopore measurements of RNA conformation can thus be useful for probing the structure of various RNA motifs, as well as structural changes to the RNA upon small-molecule binding."

For more information on this research see: Nanopore-Based Conformational Analysis of a Viral RNA Drug Target. ACS Nano, 2014;8(6):6425-6430. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; ACS Nano -

The news editors report that additional information may be obtained by contacting C. Shasha, University of California, Dept. of Chem & Biochem, La Jolla, CA 92093, United States. Additional authors for this research include R.Y. Henley, D.H. Stoloff, K.D. Rynearson, T. Hermann and M. Wanunu (see also Viral RNA).

Keywords for this news article include: La Jolla, Viral RNA, California, United States, North and Central America

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Source: Life Science Weekly

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