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Researchers at Catholic University of Korea Report New Data on Helicobacter pylori (Dual-priming oligonucleotide-based multiplex PCR using tissue...

August 4, 2014



Researchers at Catholic University of Korea Report New Data on Helicobacter pylori (Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection)

By a News Reporter-Staff News Editor at Gastroenterology Week -- Investigators publish new report on Gram-Negative Bacteria. According to news reporting out of Suwon, South Korea, by NewsRx editors, research stated, "To investigate whether tissue samples processed by the rapid urease test (RUT) kit are suitable for dual-priming oligonucleotide-based multiplex polymerase chain reaction (DPO-PCR) to detect Helicobacter pylori (H. pylori). A total of 54 patients with specific gastrointestinal symptom were enrolled in this study."

Our news journalists obtained a quote from the research from the Catholic University of Korea, "During endoscopy, gastric biopsy specimens were taken for histology, RUT, and DPO-PCR. DPO-PCR was performed on gastric biopsy samples and tissue samples that were analyzed by RUT at 2 separate institutes. In detecting H. pylori, the concordance rate of the DPO-PCR tests between the tissue samples that had been submitted to RUT and the gastric biopsy samples was investigated. H. pylori co-occurred with 76.0% (19/25) of gastric ulcers, 64.3% (9/14) of duodenal ulcers, and 33.3% (4/12) of gastritis cases. H. pylori infection was found in 100% (3/3) of the patients with both gastric and duodenal ulcers. Overall, H. pylori was detected in 35 of 54 (64.8%) patients. The diagnostic sensitivities of histology, RUT, and DPO-PCR were 85.7% (30/35), 74.3% (26/35), and 97.1% (34/35), respectively (P = 0.02). The positive predictive value (PPV) of DPO-PCR was 94.4%, whereas the negative predictive value (NPV) was 94.7%. In the rapid urease test (CLOtest)-negative cases, the frequency of positive DPO-PCR and histologic results was 20.0% (7/35). The concordance rate of the DPO-PCR tests between the tissue samples from the RUT kit and the gastric biopsy samples was 94.4% (51/54). The rate of DPO-PCR and silver stain positivity in the RUT-negative cases was 20.0% (7/35). In diagnosing H. pylori infection, DPO-PCR can be performed on tissue samples that have been processed by the RUT kit."

According to the news editors, the research concluded: "Particularly, in patients with RUT-negative results, DPO-PCR on these tissue samples could be helpful in detecting of H. pylori infection."

For more information on this research see: Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection. World Journal of Gastroenterology, 2014;20(21):6547-6553. World Journal of Gastroenterology can be contacted at: Baishideng Publishing Group Inc, 8226 Regency Dr, Pleasanton, CA 94588, USA. (Baishideng Publishing Group - www.wjgnet.com/; World Journal of Gastroenterology - www.wjgnet.com/1007-9327/current.htm)

Our news journalists report that additional information may be obtained by contacting W.C. Chung, Catholic University of Korea, St Vincent Hosp, Res Inst, Suwon 442723, South Korea. Additional authors for this research include S.H. Jung, J.H. Oh, T.H. Kim, D.Y. Cheung, B.W. Kim, S.S. Kim, J.I. Kim and E.Y. Sin (see also Gram-Negative Bacteria).

Keywords for this news article include: Asia, Suwon, Urease, Diagnosis, Histology, South Korea, Diagnostics, Amidohydrolases, Helicobacter pylori, Enzymes and Coenzymes, Epsilonproteobacteria, Helicobacter Infections, Gram-Negative Bacterial Infections

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Gastroenterology Week


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