Research Conducted at University of London Imperial College Has Provided New Information about Enzymes and Coenzymes (Label-Free Multimodal Protease Detection Based on Protein/Perylene Dye Coassembly and Enzyme-Triggered Disassembly)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Enzymes and Coenzymes. According to news reporting from London, United Kingdom, by NewsRx journalists, research stated, "The development of novel assays for protease sensing plays an important role in clinical diagnostics and therapeutics. Herein, we report a supramolecular platform for label-free protease detection, based on protein/dye self-assembly and enzyme-triggered disassembly."
The news correspondents obtained a quote from the research from the University of London Imperial College, "In a typical case, coassembly of protamine sulfate and perylene dye via electrostatic attractions and pi-pi interactions caused significant colorimetric and fluorescent responses. Subsequent addition of trypsin was found to cleave the amide bonds of protein, triggering the dissociation of protein/dye aggregates and the release of perylene dyes. The enzyme-triggered disassembly was transduced into multiple readouts including absorption, fluorescence, and polarization, which were exploited for trypsin detection and inhibitor testing. This assay was also used for turn-on fluorescence detection of cathepsin B, an enzyme known to be overexpressed in mammalian cancer cells. The integration of supramolecular self-assembly into enzyme detection in this work has provided a novel label-free biosensing platform which is highly sensitive with multimodal readouts."
According to the news reporters, the research concluded: "The relative simplicity of the approach avoids the need for time-consuming substrate synthesis, and is also amenable to naked eye detection."
For more information on this research see: Label-Free Multimodal Protease Detection Based on Protein/Perylene Dye Coassembly and Enzyme-Triggered Disassembly. Analytical Chemistry, 2014;86(13):6410-6417. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting Y.Y. Lin, University of London Imperial College, Inst Biomed Engn, London SW7 2AZ, United Kingdom. Additional authors for this research include R. Chapman and M.M. Stevens (see also Enzymes and Coenzymes).
Keywords for this news article include: London, Europe, Protease, United Kingdom, Nanotechnology, Supramolecular, Emerging Technologies, Enzymes and Coenzymes
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