Recent Findings from University of Pennsylvania Provides New Insights into Gene Therapy (Anti-inflammatory loaded poly-lactic glycolic acid nanoparticle formulations to enhance myocardial gene transfer: an in-vitro assessment of a drug/gene ...)
By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Biotechnology. According to news reporting out of Philadelphia, Pennsylvania, by NewsRx editors, research stated, "Cardiac gene therapy for heart disease is a major translational research area with potential, yet problems with safe and efficient gene transfer into cardiac muscle remain unresolved. Existing methodology to increase vector uptake include modifying the viral vector, non-viral particle encapsulation and or delivery with device systems."
Our news journalists obtained a quote from the research from the University of Pennsylvania, "These advanced methods have made improvements, however fail to address the key problem of inflammation in the myocardium, which is known to reduce vector uptake and contribute to immunogenic adverse events. Here we propose an alternative method to co-deliver anti-inflammatory drugs in a controlled release polymer with gene product to improve therapeutic effects. A robust, double emulsion production process was developed to encapsulate drugs into nanoparticles. Briefly in this proof of concept study, aspirin and prednisolone anti-inflammatory drugs were encapsulated in various poly-lactic glycolic acid polymer (PLGA) formulations. The resultant particle systems were characterized, co-delivered with GFP plasmid, and evaluated in harvested myocytes in culture for uptake. High quality nanoparticles were harvested from multiple production runs, with an average 64 +/- 10 mg yield. Four distinct particle drug system combinations were characterized and evaluated in vitro: PLGA(50: 50) Aspirin, PLGA (65: 35) Prednisolone, PLGA(65: 35) Aspirin and PLGA(50: 50) Prednisolone Particles consisted of spherical shape with a narrow size distribution 265 +/- 104 nm as found in scanning electron microscopy imaging. Prednisolone particles regardless of PLGA type were found on average approximate to 100 nm smaller than the aspirin types. All four groups demonstrated high zeta potential stability and re-constitution testing prior to in vitro. In vitro results demonstrated co uptake of GFP plasmid (green) and drug loaded particles (red) in culture with no incidence of toxicity."
According to the news editors, the research concluded: "Nano formulated anti-inflammatories in combination with standalone gene product therapy may offer a clinical solution to maximize cardiac gene therapy product effects while minimizing the risk of the host response in the inflammatory myocardial environment."
For more information on this research see: Anti-inflammatory loaded poly-lactic glycolic acid nanoparticle formulations to enhance myocardial gene transfer: an in-vitro assessment of a drug/gene combination therapeutic approach for direct injection. Journal of Translational Medicine, 2014;12():10-18. Journal of Translational Medicine can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; Journal of Translational Medicine - www.translational-medicine.com)
Our news journalists report that additional information may be obtained by contacting A.S. Fargnoli, University of Pennsylvania, Sch Engn & Appl Sci, Philadelphia, PA 19104, United States. Additional authors for this research include A.B. Mu, M.G. Katz, R.D. Williams, K.B. Margulies, D.B. Weiner, S. Yang and C.R. Bridges (see also Biotechnology).
Keywords for this news article include: Antiplatelet Agents, Biotechnology, Pharmaceuticals, Drugs, Aspirin, Cardiology, Philadelphia, Pennsylvania, Gene Therapy, Nanoparticle, United States, Benzoic Acids, Hydroxy Acids, Bioengineering, Nanotechnology, Salicylic Acids, Carboxylic Acids, Organic Chemicals, Coagulation Modifiers, Emerging Technologies
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