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New Stem Cells Study Results Reported from L.N. Niu et al (Intrafibrillar-silicified collagen scaffolds enhance the osteogenic capacity of human...

August 6, 2014

New Stem Cells Study Results Reported from L.N. Niu et al (Intrafibrillar-silicified collagen scaffolds enhance the osteogenic capacity of human dental pulp stem cells)

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Stem Cell Research have been published. According to news reporting out of Augusta, Georgia, by NewsRx editors, research stated, "The present study investigated the effects of intrafibrillar-silicified collagen scaffolds (ISCS) on the osteogenic differentiation of human dental pulp stem cells (hDPSCs) in vitro and in vivo. The hDPSCs were co-cultured with ISCS or nonsilicified collagen scaffolds (NCS) in control medium (CM) or osteogenic differentiation medium (ODM)."

Our news journalists obtained a quote from the research, "Cell cycle and cell apoptosis were analyzed with flow cytometry to measure the viability of hDPSCs. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to evaluate the expression levels of osteogenic marker genes and proteins of hDPSCs. Alkaline phosphatase (ALP) staining and alizarin red S assay were used to evaluate the ALP activity of hDPSCs and their calcium deposition potential. In addition, hDPSCs and scaffolds were implanted subcutaneously in nude mice for 8 weeks. Harvested tissues were immunohistochemically stained for osteocalcin (OCN) expression from hDPSCs, and stained with alizarin red S for examination of their calcium deposition in vivo. The ISCS had no adverse effect on hDPSCs, promoted their proliferation, and significantly up-regulated the expression of osteogenesis-related genes and proteins. The hDPSCs co-cultured with ISCS in ODM exhibited the highest ALP activity and calcium deposition in vitro. The ISCS promoted the OCN expression and calcium deposition of hDPSCs after ectopic transplantation in vivo. Intrafibrillar-silicified collagen scaffolds significantly promoted the proliferation, osteogenic differentiation and mineralization of hDPSCs, when compared with NCS."

According to the news editors, the research concluded: "This study demonstrates combining the use of hDPSCs and ISCS to promote bone-like tissue formation is a promising approach for clinical bone repair and regeneration."

For more information on this research see: Intrafibrillar-silicified collagen scaffolds enhance the osteogenic capacity of human dental pulp stem cells. Journal of Dentistry, 2014;42(7):839-849. Journal of Dentistry can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier -; Journal of Dentistry -

Our news journalists report that additional information may be obtained by contacting L.N. Niu, Georgia Regents Univ, Coll Dental Med, Dept. of Endodont, Augusta, GA 30912, United States. Additional authors for this research include J.Q. Sun, Q.H. Li, K. Jiao, L.J. Shen, D. Wu, F. Tay and J.H. Chen (see also Stem Cell Research).

Keywords for this news article include: Tissue Engineering, Biomedical Engineering, Biomedicine, Augusta, Georgia, Collagen, Dentistry, United States, Bone Research, Bioengineering, Stem Cell Research, North and Central America, Extracellular Matrix Proteins

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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