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New Staphylococcus aureus Findings from University of Arkansas Outlined (Detection of Staphylococcus Aureus using quantum clots as fluorescence...

August 5, 2014



New Staphylococcus aureus Findings from University of Arkansas Outlined (Detection of Staphylococcus Aureus using quantum clots as fluorescence labels)

By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Gram-Positive Bacteria is now available. According to news reporting originating in Fayetteville, Arkansas, by NewsRx journalists, research stated, "Staphylococcus aureus (S. aureus) has been identified as one of the major foodborne pathogenic bacteria. The development of rapid detection methods for S. aureus is needed for assuring food safety."

The news reporters obtained a quote from the research from the University of Arkansas, "In this study, quantum dots were used as fluorescent labels in an immunoassay for quantitative detection of S. aureus. Firstly, biotin-labeled anti-S. aureus antibody was conjugated with streptavidin-coated magnetic nanobeads (180 mu diameter) and used to separate S. aureus cells. Then streptavidin coated quantum dots (QDs) were conjugated with biotin-labeled anti-S. aureus antibody and used as the fluorescence labels to mix with the separated S. aureus. Finally the fluorescence intensity of the bead-cell-QD complexes was measured at a wavelength of 620 mu. A linear relationship between S. aureus cell number (X) and fluorescence intensity (Y) was found for cell numbers ranging from 10(3) to 10(6) CFU (Colony Forming Unit)/mL, and the detection limit was 10(3) CFU/mL. The regression model can be expressed as Y = 7.68X + 35.06 with R-2=0.94. The detection of S. aureus in food sample was explored initially. The fluorescence intensity of food sample was close to the background, so it was not satisfied."

According to the news reporters, the research concluded: "Further study will focus on the application of the method for detection of S. aureus in food sample."

For more information on this research see: Detection of Staphylococcus Aureus using quantum clots as fluorescence labels. International Journal of Agricultural and Biological Engineering, 2014;7(1):77-83. International Journal of Agricultural and Biological Engineering can be contacted at: Chinese Acad Agricultural Engineering, Rm 506, 41, Maizidian St, Chaoyang District, Beijing, 100125, Peoples R China (see also Gram-Positive Bacteria).

Our news correspondents report that additional information may be obtained by contacting Y.H. Hu, University of Arkansas, Dept. of Biol & Agr Engn, Fayetteville, AR 72701, United States. Additional authors for this research include C.C. Wang, B. Bing, M.T. Li, R.H. Wang and Y.B. Li.

Keywords for this news article include: Arkansas, Bacillales, Fayetteville, Quantum Dots, United States, Nanotechnology, Quantum Physics, Staphylococcaceae, Gram-Positive Cocci, Emerging Technologies, Staphylococcus aureus, Gram-Positive Bacteria, North and Central America, Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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