New Nanoparticles Study Findings Have Been Reported by Investigators at University of Isfahan (Electrochemical Genotyping of Single-Nucleotide Polymorphisms by using Monobase-Conjugated Modified Nanoparticles)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Nanoparticles. According to news reporting from Esfahan, Iran, by NewsRx journalists, research stated, "Currently, construction of DNA biosensors for single-nucleotide polymorphisms (SNPs) genotyping is challenging, particularly in terms of speed and cost. We demonstrate a single platform for genotyping the SNPs by using electrochemical signals of modified nanoparticles (MNPs)."
The news correspondents obtained a quote from the research from the University of Isfahan, "To this end, silver and gold nanoparticles (AgNPs and AuNPs) are modified by using cysteine and cysteamine hydrochloride as linkers, respectively. The monobases are subsequently attached to the MNPs through their 5' phosphate group, forming a phosphoramidate bond with free amino groups of the linkers. Electrooxidation signals of AgNPs and/or 3, 4-diaminobenzoic acid (DABA) are monitored as analytical signals for SNP genotyping. In the presence of DNA polymerase I (Klenow fragment), the coupling of monobase-conjugated MNPs (MMNPs) is induced to the mutant sites of duplex DNA, following the Watson-Crick base-pairing rule, which leads to a substantial change in the signal intensity of MMNPs. The method is able to distinguish complementary targets with a linear dynamic range of 20-1000 pm and 50-1500 pm of mutant DNA, with reliable reproducibility [relative standard deviation (RSD)=5.1% and stability (RSD=4.2 %)."
According to the news reporters, the research concluded: "In this study, an accurate, sensitive, fast, and cost-effective approach for SNP genotyping is introduced."
For more information on this research see: Electrochemical Genotyping of Single-Nucleotide Polymorphisms by using Monobase-Conjugated Modified Nanoparticles. Chemelectrochem, 2014;1(4):779-786. Chemelectrochem can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany (see also Nanoparticles).
Our news journalists report that additional information may be obtained by contacting S.M. Khoshfetrat, Univ Isfahan, Dept. of Chem, Esfahan 8174673441, Iran.
Keywords for this news article include: Iran, Asia, Esfahan, Chemistry, Nanotechnology, Electrochemical, Emerging Technologies
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