New Glycoside Hydrolases Findings from Dalian Institute of Chemical Physics Outlined (Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Enzymes and Coenzymes have been published. According to news reporting originating from Dalian, People's Republic of China, by NewsRx correspondents, research stated, "Small molecule inhibitors against chitinases have potential applications as pesticides, fungicides, and antiasthmatics. Here, we report that a series of fully deacetylated chitooligosaccharides (GlcN)(2-7) can act as inhibitors against the insect chitinase OfChtI, the human chitinase HsCht, and the bacterial chitinases SmChiA and SmChiB with IC50 values at micromolar to millimolar levels."
Our news editors obtained a quote from the research from the Dalian Institute of Chemical Physics, "The injection of mixed (GlcN)(2-7) into the fifth instar larvae of the insect Ostrinia furnacalis resulted in 85% of the larvae being arrested at the larval stage and death after 10 days, also suggesting that (GlcN)(2-7) might inhibit OfChtI in vivo. Crystal structures of the catalytic domain of OfChtI (OfChtI-CAD) complexed with (GlcN)(5,6) were obtained at resolutions of 2.0 angstrom. These structures, together with mutagenesis and thermodynamic analysis, suggested that the inhibition was strongly related to the interaction between the -1 GlcN residue of the inhibitor and the catalytic Glu(148) of the enzyme. Structure-based comparison showed that the fully deacetylated chitooligosaccharides mimic the substrate chitooligosaccharides by binding to the active cleft. This work first reports the inhibitory activity and proposed inhibitory mechanism of fully deacetylated chitooligosaccharides."
According to the news editors, the research concluded: "Because the fully deacetylated chitooligosaccharides can be easily derived from chitin, one of the most abundant materials in nature, this work also provides a platform for developing eco-friendly inhibitors against chitinases."
For more information on this research see: Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors. Journal of Biological Chemistry, 2014;289(25):17932-17940. Journal of Biological Chemistry can be contacted at: Amer Soc Biochemistry Molecular Biology Inc, 9650 Rockville Pike, Bethesda, MD 20814-3996, USA. (American Society for Biochemistry and Molecular Biology - www.asbmb.org; Journal of Biological Chemistry - www.jbc.org/)
The news editors report that additional information may be obtained by contacting L. Chen, Chinese Academy Sci, Dalian Inst Chem Phys, Dalian 116023, People's Republic of China. Additional authors for this research include Y. Zhou, M.B. Qu, Y. Zhao and Q. Yang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Dalian, Chitinase, Glycoside Hydrolases, Enzymes and Coenzymes, People's Republic of China
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