New Findings Reported from Southwest University Describe Advances in Heme (A pseudo triple-enzyme electrochemical aptasensor based on the amplification of Pt-Pd nanowires and hemin/G-quadruplex)
By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biological Factors. According to news originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "Our present work aimed at developing a pseudo triple-enzyme cascade electrocatalytic electrochemical aptasensor for determination of thrombin with the amplification of alcohol dehydrogenase (ADH)-Pt-Pd nanowires bionanocomposite and hemin/G-quadruplex structure that simultaneously acted as NADH oxidase and HRP-mimicking DNAzyme. With the addition of ethanol to the electrolyte, the ADH immobilized on the Pt-Pd nanowires catalyzed ethanol to acetaldehyde accompanied by NAD(+) being converted to NADH."
Our news journalists obtained a quote from the research from Southwest University, "Then the hemin/G-quadruplex firstly served as NADH oxidase, converting the produced NADH to NAD(+) with the concomitant local formation of high concentration of H2O2. Subsequently, the hemin/G-quadruplex acted as HRP-mimicking DNAzyme, bioelectrocatalyzing the produced H2O2. At the same time, the Pt-Pd nanowires employed in our strategy not only provided a large surface area for immobilizing thrombin binding aptamer (TBA) and ADH, but also served as HRP-mimicking DNAzyme which rapidly bioelectrocatalyzed the reduction of the produced H2O2. Thus, such a pseudo triple-enzyme cascade electrochemical aptasensor could greatly promote the electron transfer of hemin and resulted in the dramatic enhancement of electrochemical signal. As a result, a wide dynamic concentration linear range from 0.2 pM to 20 nM with a low detection limit of 0.067 pM for thrombin (TB) determination was obtained."
According to the news editors, the research concluded: "The excellent performance indicated that our strategy was a promising way for ultrasensitive assays in electrochemical aptasensors."
For more information on this research see: A pseudo triple-enzyme electrochemical aptasensor based on the amplification of Pt-Pd nanowires and hemin/G-quadruplex. Analytica Chimica Acta, 2014;834():45-50. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news correspondents report that additional information may be obtained from Y.N. Zheng, Southwest University, Coll Chem & Chem Engn, Key Lab Luminescence & Real Time Anal, Minist Educ, Chongqing 400715, People's Republic of China. Additional authors for this research include Y.Q. Chai, Y.L. Yuan and R. Yuan (see also Biological Factors).
Keywords for this news article include: Asia, Heme, Drugs, Oxidase, Therapy, Nanowire, Thrombin, Chongqing, Chemistry, Porphyrins, Nanotechnology, Electrochemical, Biological Factors, Emerging Technologies, Enzymes and Coenzymes, Blood Coagulation Factors, People's Republic of China
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