New Findings from University of Pittsburgh in Tissue Engineering Provides New Insights (Scaffold attachment factor B1 regulates the androgen receptor in concert with the growth inhibitory kinase MST1 and the methyltransferase EZH2)
By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biomedicine and Biomedical Engineering. According to news reporting originating from Pittsburgh, Pennsylvania, by NewsRx correspondents, research stated, "The androgen receptor (AR) is a transcription factor that employs many diverse interactions with coregulatory proteins in normal physiology and in prostate cancer (PCa). The AR mediates cellular responses in association with chromatin complexes and kinase cascades."
Our news editors obtained a quote from the research from the University of Pittsburgh, "Here we report that the nuclear matrix protein, scaffold attachment factor B1 (SAFB1), regulates AR activity and AR levels in a manner that suggests its involvement in PCa. SAFB1 mRNA expression was lower in PCa in comparison with normal prostate tissue in a majority of publicly available RNA expression data sets. SAFB1 protein levels were also reduced with disease progression in a cohort of human PCa that included metastatic tumors. SAFB1 bound to AR and was phosphorylated by the MST1 (Hippo homolog) serine-threonine kinase, previously shown to be an AR repressor, and MST1 localization to AR-dependent promoters was inhibited by SAFB1 depletion. Knockdown of SAFB1 in androgen-dependent LNCaP PCa cells increased AR and prostate-specific antigen (PSA) levels, stimulated growth of cultured cells and subcutaneous xenografts and promoted a more aggressive phenotype, consistent with a repressive AR regulatory function. SAFB1 formed a complex with the histone methyltransferase EZH2 at AR-interacting chromatin sites in association with other polycomb repressive complex 2 (PRC2) proteins."
According to the news editors, the research concluded: "SAFB1 acts as a novel AR co-regulator at gene loci where signals from the MST1/Hippo and EZH2 pathways converge."
For more information on this research see: Scaffold attachment factor B1 regulates the androgen receptor in concert with the growth inhibitory kinase MST1 and the methyltransferase EZH2. Oncogene, 2014;33(25):3235-3245. Oncogene can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group - www.nature.com/; Oncogene - www.nature.com/onc/)
The news editors report that additional information may be obtained by contacting N.K. Mukhopadhyay, University of Pittsburgh, Dept. of Pharmacol & Chem Biol, Pittsburgh, PA, United States. Additional authors for this research include J. Kim, S. You, M. Morello, M.H. Hager, W.C. Huang, A. Ramachandran, J. Yang, B. Cinar, M.A. Rubin, R.M. Adam, S. Oesterreich, D. Di Vizio and M.R. Freeman (see also Biomedicine and Biomedical Engineering).
Keywords for this news article include: Tissue Engineering, Biomedicine and Biomedical Engineering, Kinase, Pittsburgh, Pennsylvania, United States, Bioengineering, Steroid Receptors, Androgen Receptors, Methyltransferases, DNA-Binding Proteins, Enzymes and Coenzymes, Transcription Factors, North and Central America, One-Carbon Group Transferases
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