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New Findings from Shahed University in the Area of Neurotoxins Described (High level expression, purification and immunogenicity analysis of a...

August 8, 2014



New Findings from Shahed University in the Area of Neurotoxins Described (High level expression, purification and immunogenicity analysis of a protective recombinant protein against botulinum neurotoxin type E)

By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Neurotoxins. According to news reporting from Tehran, Iran, by NewsRx journalists, research stated, "Botulinum neurotoxin type E heavy chain consists of two domains: N-terminal half as a translocation domain and C-terminal half (Hcc) as a binding domain. In this research a synthetic gene fragment encoding the binding domain of botulinum neurotoxin type E (BoNT/E-Hcc) was highly expressed in Escherichia coli by pGEX4T-1 vector."

The news correspondents obtained a quote from the research from Shahed University, "After purification, the recombinant BoNT/E-Hcc was evaluated by SDS-PAGE and western blot (immunoblot) analysis. Average yields obtained in this research were 3.7 mg recombinant BoNT/E-Hcc per liter of bacterial culture. The recombinant protein was injected in mice for study of its protection ability against botulinum neurotoxin type E challenges."

According to the news reporters, the research concluded: "The challenge studies showed that, vaccinated mice were fully protected against 10 4 9 minimum lethal dose of botulinum neurotoxin type E."

For more information on this research see: High level expression, purification and immunogenicity analysis of a protective recombinant protein against botulinum neurotoxin type E. World Journal of Microbiology & Biotechnology, 2014;30(6):1861-1867. World Journal of Microbiology & Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA (see also Neurotoxins).

Our news journalists report that additional information may be obtained by contacting E. Valipour, Shahed University, Fac Sci, Dept. of Biol, Tehran, Iran. Additional authors for this research include M.L. Moosavi, J. Amani and S. Nazarian.

Keywords for this news article include: Iran, Asia, Tehran, Neurotoxins, Recombinant Proteins

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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