New Findings from School of Civil and Environmental Engineering in DNA Research Provides New Insights (Examination of Nanoparticle-DNA Binding Characteristics Using Single-Molecule Imaging Atomic Force Microscopy)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in DNA Research. According to news reporting out of Atlanta, Georgia, by NewsRx editors, research stated, "Understanding the characteristics of nanoparticle (NP)-to-DNA binding is important for the rational design of functionalized NPs to use in biomedical applications as well as for the toxicological assessment of NPs. In this study we employed a single-molecule imaging technique, atomic force microscopy (AFM), to determine the characteristics of NP-to-DNA binding, including the binding kinetics, isotherm, and specificity."
Our news journalists obtained a quote from the research from the School of Civil and Environmental Engineering, "We demonstrated the capability of this AFM-based approach using quantum dots (QDs) as a model NP. The binding kinetics and binding isotherm of QDs to DNA were investigated by examining a large number of single DNA molecules after exposure to QDs using AFM; the models that we developed fit the experimental results well. According to the binding kinetics model, the average number of bound QDs per DNA molecule at equilibrium is approximately five, and the binding rate constant is approximately 0.35 s(-1). Furthermore, from the binding isotherm, the equilibrium binding constant and maximum number of QDs bound to DNA were determined to be approximately 0.23 nM(-1) and 14, respectively."
According to the news editors, the research concluded: "Finally, by examining the position of QDs on DNA molecules, i.e., the distance from a QD to the nearest DNA terminus, we found that the binding of QDs to DNA is nonspecific."
For more information on this research see: Examination of Nanoparticle-DNA Binding Characteristics Using Single-Molecule Imaging Atomic Force Microscopy. Journal of Physical Chemistry C, 2014;118(25):13876-13882. Journal of Physical Chemistry C can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Physical Chemistry C - www.pubs.acs.org/journal/jpccck)
Our news journalists report that additional information may be obtained by contacting K.G. Li, Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332, United States (see also DNA Research).
Keywords for this news article include: Atlanta, Georgia, DNA Research, United States, Atomic Force Microscopy, North and Central America
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