New Findings from Institute of Materials Research and Engineering in the Area of DNA Research Described (Interrogating Cooperative Interactions of Transcription Factors with Composite DNA Elements Using Gold Nanoparticles)
By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on DNA Research have been presented. According to news reporting out of Singapore, Singapore, by NewsRx editors, research stated, "We have developed a colorimetric assay using gold nanoparticles (AuNPs) to assess the cooperative binding interactions between two transcription factors related to breast cancer development, i.e., estrogen receptor (ER) and specificity protein 1 (Sp1), with composite DNA elements. The composite DNA contains an estrogen response element (ERE) half-site and Sp1/GC-rich binding sites with a four nucleotides spacer."
Our news journalists obtained a quote from the research from the Institute of Materials Research and Engineering, "The assay design was conceptualised based on our previous findings that the size of protein-DNA complex is the determining factor to stabilize citrate anion-capped AuNPs from salt-induced aggregation, demonstrated using binary ER-ERE complexes. In this study, we have further proven that this concept is effective for detecting ternary protein-DNA complex of ER/Sp1-DNA, where the multiprotein-DNA complexes foster a better particle stabilization effect than the ER-DNA complex and/or protein (ER or Sp1) alone due predominantly to their distinct size differences. Based on the particle size and/or interparticle-distance dependent color and UV-vis spectroscopic properties of AuNPs, the formation of a ternary complex between ER alpha, Sp1, and the half-ERE/Sp1 composite element in the promoter A of the human PR gene can be detected. With this assay, we have also confirmed the cooperative binding of ER beta with Sp1, which has been less well characterized before in term of their cooperative effect in estrogen regulation."
According to the news editors, the research concluded: "This homogenous 'mix-and-test' AuNPs assay is easy to use that can facilitate further understanding on how ER and Sp1 influence and activate gene transcription by forming cooperative binding compound to DNA, a key aspect in breast cancer biology."
For more information on this research see: Interrogating Cooperative Interactions of Transcription Factors with Composite DNA Elements Using Gold Nanoparticles. Science of Advanced Materials, 2014;6(7):1460-1466. Science of Advanced Materials can be contacted at: Amer Scientific Publishers, 26650 The Old Rd, Ste 208, Valencia, CA 91381-0751, USA. (American Scientific Publishers - www.aspbs.com/)
Our news journalists report that additional information may be obtained by contacting Y.N. Tan, ASTAR, Inst Mat Res & Engn, Singapore 117602, Singapore. Additional authors for this research include A. Lai and X.D. Su (see also DNA Research).
Keywords for this news article include: Asia, DNA Research, Nanoparticle, Nanotechnology, Emerging Technologies, Transcription Factors
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