New Escherichia Coli Findings Reported from Anhui Agricultural University (Engineering uroporphyrinogen III methyltransferase as a red fluorescent reporter in E. coli)
By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteobacteria are discussed in a new report. According to news reporting from Anhui, People's Republic of China, by NewsRx journalists, research stated, "Uroporphyrinogen III methyltransferase (UMT) is a novel reporter owing to the catalytic products in the cells that emit strong red fluorescence under UV light. Here, we engineered the gene encoding the functional barley UMT (bUMT) by error-prone PCR and broadened the application UMT as a red fluorescent reporter in Escherichia coli."
The news correspondents obtained a quote from the research from Anhui Agricultural University, "A variant, termed mbUMT, was selected and emitted stronger cell fluorescence than the wild type bUMT expressed in different E. coil strains, under different promoters and induction conditions respectively. The constructed mbUMT with a C-terminal ssrA tag was degraded in cells by the protease ClpXP encoded by E. coli chromosome, whereas the bUMT was expressed as active aggregates. Before they are exported to the periplasm, both proteins catalyze the substrate in the cytoplasm and emit cell fluorescence."
According to the news reporters, the research concluded: "The results suggested that the evolved bUMT is a better candidate to monitor in vivo degradation by E. coli ClpXP."
For more information on this research see: Engineering uroporphyrinogen III methyltransferase as a red fluorescent reporter in E. coli. Enzyme and Microbial Technology, 2014;61-62():1-6. Enzyme and Microbial Technology can be contacted at: Elsevier Science Inc, 360 Park Ave South, New York, NY 10010-1710, USA. (Elsevier - www.elsevier.com; Enzyme and Microbial Technology - www.elsevier.com/wps/product/cws_home/525004)
Our news journalists report that additional information may be obtained by contacting Z.Z. Wang, Anhui Agr Univ, Sch Life Sci, Hefei 230036, Anhui, People's Republic of China. Additional authors for this research include S. Li, J. Li, J.J. Li, L. Rong, B.J. Cheng and J. Fan (see also Proteobacteria).
Keywords for this news article include: Asia, Anhui, Engineering, Escherichia, Uroporphyrinogens, Enterobacteriaceae, Gammaproteobacteria, People's Republic of China
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