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New Biomedicine and Biomedical Engineering Findings Reported from G.L. Thompson and Co-Authors (Calcium influx affects intracellular transport and...

August 6, 2014



New Biomedicine and Biomedical Engineering Findings Reported from G.L. Thompson and Co-Authors (Calcium influx affects intracellular transport and membrane repair following nanosecond pulsed electric field exposure)

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting from Fort Sam Houston, Texas, by NewsRx journalists, research stated, "The cellular response to subtle membrane damage following exposure to nanosecond pulsed electric fields (nsPEF) is not well understood. Recent work has shown that when cells are exposed to nsPEF, ion permeable nanopores ( 2 nm) created by longer micro- and millisecond duration pulses."

The news correspondents obtained a quote from the research, "Nanoporation of the plasma membrane by nsPEF has been shown to cause a transient increase in intracellular calcium concentration within milliseconds after exposure. Our research objective is to determine the impact of nsPEF on calcium-dependent structural and repair systems in mammalian cells. Chinese hamster ovary (CHO-K1) cells were exposed in the presence and absence of calcium ions in the outside buffer to either 1 or 20, 600-ns duration electrical pulses at 16.2 kV/cm, and pore size was determined using propidium iodide and calcium green. Membrane organization was observed with morphological changes and increases in FM1-43 fluorescence. Migration of lysosomes, implicated in membrane repair, was followed using confocal microscopy of red fluorescent protein-tagged LAMP1. Microtubule structure was imaged using mEmerald-tubulin."

According to the news reporters, the research concluded: "We found that at high 600-ns PEF dosage, calcium-induced membrane restructuring and microtubule depolymerization coincide with interruption of membrane repair via lysosomal exocytosis."

For more information on this research see: Calcium influx affects intracellular transport and membrane repair following nanosecond pulsed electric field exposure. Journal of Biomedical Optics, 2014;19(5):115-126. Journal of Biomedical Optics can be contacted at: Spie-Soc Photo-Optical Instrumentation Engineers, 1000 20TH St, PO Box 10, Bellingham, WA 98225, USA (see also Biotechnology).

Our news journalists report that additional information may be obtained by contacting G.L. Thompson, JBSA Ft Sam Houston, Fort Sam Houston, TX 78234, United States. Additional authors for this research include C.C. Roth, D.R. Dalzell, M. Kuipers and B.L. Ibey.

Keywords for this news article include: Biotechnology, Texas, United States, Fort Sam Houston, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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