Investigators from School of Life Science and Technology Zero in on Coenzymes (Recognition Kinetics of Biomolecules at the Surface of Different-Sized Spheres)
By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Enzymes and Coenzymes is the subject of a report. According to news reporting out of Beijing, People's Republic of China, by NewsRx editors, research stated, "Bead-based assay is widely used in many bioanalytical applications involving the attachment of proteins and other biomolecules to the surface. For further understanding of the formation of a sphere-biomolecule complex and easily optimizing the use of spheres in targeted biological applications, it is necessary to know the kinetics of the binding reaction at sphere/solution interface."
Our news journalists obtained a quote from the research from the School of Life Science and Technology, "In our presented work, a simple fluorescence analysis method was employed to measure the kinetics for the binding of biotin to sphere surface-bound FITC-SA, based on the fact that the fluorescence intensity of FITC was proportionally enhanced by increasing the binding amount of biotin. By monitoring the time-dependent changes of FITC fluorescence, it was found that the binding rate constant of biotin to sphere surface-immobilized FITC-SA was much smaller than that of biotin to freely diffusing FITC-SA. This can be attributed to the decreased encounter frequency of the reaction pair, restricted motion of the attached biomolecule, and the weakened steric accessibility of the binding site. These factors would become more obvious when increasing the size of the sphere upon which the FITC-SA was immobilized."
According to the news editors, the research concluded: "Additionally, the effect of nanoparticles on the diffusion-controlled bimolecular binding reaction was more evident than that on the chemical recognition-controlled binding reaction."
For more information on this research see: Recognition Kinetics of Biomolecules at the Surface of Different-Sized Spheres. Biophysical Journal, 2014;107(1):165-173. Biophysical Journal can be contacted at: Cell Press, 600 Technology Square, 5TH Floor, Cambridge, MA 02139, USA. (Elsevier - www.elsevier.com; Biophysical Journal - www.elsevier.com/wps/product/cws_home/716950)
Our news journalists report that additional information may be obtained by contacting J. Hu, Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, People's Republic of China. Additional authors for this research include C.Y. Wen, Z.L. Zhang, M. Xie, H.Y. Xie and D.W. Pang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Biotin, Beijing, Micronutrient, Diet and Nutrition, Enzymes and Coenzymes, People's Republic of China
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