Investigators from Hanyang University Zero in on Stem Cells (In Vivo Differentiation of Undifferentiated Human Adipose Tissue-Derived Mesenchymal Stem Cells in Critical-Sized Calvarial Bone Defects)
By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Stem Cell Research have been presented. According to news reporting out of Seoul, South Korea, by NewsRx editors, research stated, "Adult stem cells have recently drawn considerable attention for potential cell therapy applications. However, critical details about their specific in vivo environments and cellular activities are unclear."
Our news journalists obtained a quote from the research from Hanyang University, "Adipose tissue-derived mesenchymal stem cells (ASCs) are attractive candidates for treating bone defects, but most studies focus on delivery of in vitro-differentiated cells. We assessed various scaffolding materials for the ability to support osteogenic differentiation of undifferentiated human ASCs in vivo, in athymic nude rat calvaria. Twenty-four 9- to 10-week-old athymic nude Sprague-Dawley rats (250 g) were used in these experiments. Fat tissue from 3 patients was harvested from abdominal tissue discarded during reconstructive breast surgery by transverse rectus abdominis myocutaneous flap, performed at the Asan Medical Center after resection of breast cancer. Human ASCs were extracted from discarded adipose tissue and isolated based on standard International Society for Cellular Therapy protocols. Adipose tissue-derived mesenchymal stem cells were seeded on polylactic glycolic acid, atelocollagen, and hydroxyapatite scaffolds, and osteogenesis was evaluated using bone mineral densitometry, histology, immunohistochemistry, and reverse transcription polymerase chain reaction. The gross appearance of scaffolds seeded with ASCs was strikingly different from that of scaffolds alone. Bone mineral densitometry analysis revealed a 2- to 3-fold increase in mineral density in ASC-seeded scaffolds. In addition, undifferentiated ASCs seeded onto hydroxyapatite scaffolds, but not onto collagen or polylactic glycolic acid scaffolds, expressed human messenger RNA for osteogenic markers such as alkaline phosphatase, osteopontin, osteocalcin, and osteonectin."
According to the news editors, the research concluded: "These results indicate that undifferentiated human ASCs can differentiate into osteocytes or osteoblasts in athymic nude rat calvaria, and the importance of appropriate scaffolding for in vivo ASC differentiation."
For more information on this research see: In Vivo Differentiation of Undifferentiated Human Adipose Tissue-Derived Mesenchymal Stem Cells in Critical-Sized Calvarial Bone Defects. Annals of Plastic Surgery, 2014;72(2):225-233. Annals of Plastic Surgery can be contacted at: Lippincott Williams & Wilkins, 530 Walnut St, Philadelphia, PA 19106-3621, USA. (Lippincott Williams and Wilkins - www.lww.com; Annals of Plastic Surgery - journals.lww.com/annalsplasticsurgery/pages/default.aspx)
Our news journalists report that additional information may be obtained by contacting J.W. Choi, Hanyang University, Dept. of Biomed Engn, Seoul 133791, South Korea. Additional authors for this research include E.J. Park, H.S. Shin, I.S. Shin, J.C. Ra and K.S. Koh (see also Stem Cell Research).
Keywords for this news article include: Asia, Tissue Engineering, Biomedical Engineering, Biomedicine, Seoul, South Korea, Bone Research, Bioengineering, Stem Cell Research, Mesenchymal Stem Cells
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