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Investigators at German Cancer Research Center Zero in on DNA Research (A bead-based multiplex assay for the detection of DNA viruses infecting...

August 5, 2014



Investigators at German Cancer Research Center Zero in on DNA Research (A bead-based multiplex assay for the detection of DNA viruses infecting laboratory rodents)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting out of Heidelberg, Germany, by NewsRx editors, research stated, "The Federation of European Laboratory Animal Science Association (FELASA) recommends screening of laboratory rodents and biological materials for a broad variety of bacterial agents, viruses, and parasites. Methods commonly used to date for pathogen detection are neither cost-effective nor time-and animal-efficient or uniform."

Our news journalists obtained a quote from the research from German Cancer Research Center, "However, an infection even if silent alters experimental results through changing the animals' physiology and increases inter-individual variability. As a consequence higher numbers of animals and experiments are needed for valid and significant results. We developed a novel high-throughput multiplex assay, called rodent DNA virus finder (rDVF) for the simultaneous identification of 24 DNA viruses infecting mice and rats. We detected all 24 DNA viruses with high specificity and reproducibility. Detection limits for the different DNA viruses varied between 10 and 1000 copies per PCR. The validation of rDVF was done with DNA isolated from homogenised organs amplified by pathogen specific primers in one multiplex PCR. The biotinylated amplicons were detected via hybridisation to specific oligonucleotide probes coupled to spectrally distinct sets of fluorescent Luminex beads."

According to the news editors, the research concluded: "RDVF may have the potential to replace conventional testing and may simplify and improve routine detection of DNA viruses infecting rodents."

For more information on this research see: A bead-based multiplex assay for the detection of DNA viruses infecting laboratory rodents. Plos One, 2014;9(5):e97525. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)

Our news journalists report that additional information may be obtained by contacting D. Hofler, Research Program Infection and Cancer, German Cancer Research Center, Heidelberg, Baden-Wurttemberg, Germany. Additional authors for this research include W. Nicklas, P. Mauter, M. Pawlita and M. Schmitt (see also DNA Research).

Keywords for this news article include: Europe, Germany, Viral DNA, Heidelberg, DNA Research.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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