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Findings on Nitric Oxide Reported by Researchers at National Institute of Animal Science (Reprogramming sertoli cells into pluripotent stem cells)

August 6, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Nitric Oxide. According to news reporting out of Beijing, People's Republic of China, by NewsRx editors, research stated, "Induced pluripotent stem cells (iPSCs) have potential applications in the restoration of fertility, regenerative medicine, and animal biotechnology. In this study, we present the induction of iPSCs from mouse Sertoli cells (SCs) by introducing four factors--Oct4, Sox2, Klf4, and c-Myc."

Our news journalists obtained a quote from the research from the National Institute of Animal Science, "As early as day 3 after induction, expression of these factors was detected and typical embryonic stem-like cells began to form. On day 18, these exogenous genes were silenced and colonies were selected according to morphological characteristics. The iPSCs induced from SCs, termed SCiPSCs, strongly expressed pluripotent markers, showed a normal karyotype, and had proliferation and differentiation characteristics similar to those of embryonic stem cells (ESCs), both in vitro and in vivo. Furthermore, exposure of SCiPSCs to nitric oxide (NO) allowed them to maintain pluripotency through the activation of the pluripotent genes Oct4 and Sox2 and upregulation of Nanog expression. Moreover, NO prevented SCiPSCs from undergoing apoptosis by activating the antiapoptotic genes Bcl2 and Bcl2lll, downregulating the proapoptotic genes Bak1 and Casp7, and blocking the activation of the proapoptotic gene Bac. These effects were reversed by exposure to l-NG-monomethylarginine (l-NMMA), a NO inhibitor."

According to the news editors, the research concluded: "These data demonstrate that iPSCs can be generated from SCs and that the self-renewal and pluripotency of SCiPS cells can be maintained in the presence of NO."

For more information on this research see: Reprogramming sertoli cells into pluripotent stem cells. Cellular Reprogramming, 2014;16(3):196-205. (Mary Ann Liebert, Inc. -; Cellular Reprogramming -

Our news journalists report that additional information may be obtained by contacting H. Sun, 1 Transgenic and Stem Cell Core, National Institute of Animal Science and Veterinary Medicine , Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China. Additional authors for this research include G. Zhang, F. Dong, F. Wang and W. Cao (see also Nitric Oxide).

Keywords for this news article include: Asia, Beijing, Nitric Oxide, Sertoli Cells, Endocrine Cells, People's Republic of China.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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